Evidence map›Paper›PMID 38213324›Full record

ArticleBio-protocol2024

Expansion and Polarization of Human γδT17 Cells in vitro from Peripheral Blood Mononuclear Cells.

Xu Chen, Xiaoling Hu, Fuxiang Chen, Jun Yan

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Xu ChenDepartment of Clinical Immunology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xiaoling HuDivision of Immunotherapy, The Hiram C. Polk, Jr., MD Department of Surgery, Immuno-Oncology Program, Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA.
Fuxiang ChenDepartment of Clinical Immunology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Jun YanDivision of Immunotherapy, The Hiram C. Polk, Jr., MD Department of Surgery, Immuno-Oncology Program, Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA.
University of Louisville · USShanghai Ninth People's Hospital · CN

Funding

Transcriptional Regulation of Immunosuppressive Macrophages by c-Maf in CancerR01CA213990 · NCI · UNIVERSITY OF LOUISVILLE · PI YAN, JUN · 2017 to 2021
$1.8M
Regulation of dermal gammadelta T cells by microbial pathogens/commensals in health and psoriasisR01AI128818 · NIAID · UNIVERSITY OF LOUISVILLE · PI YAN, JUN · 2017 to 2021
$974k
NCI NIH HHS R01 CA213990NIAID NIH HHS R01 AI128818
6 · The paper itself

Abstract

γδ T cells play a critical role in homeostasis and diseases such as infectious diseases and tumors in both mice and humans. They can be categorized into two main functional subsets: IFN-γ-producing γδT1 cells and IL-17-producing γδT17 cells. While CD27 expression segregates these two subsets in mice, little is known about human γδT17 cell differentiation and expansion. Previous studies have identified γδT17 cells in human skin and mucosal tissues, including the oral cavity and colon. However, human γδ T cells from peripheral blood mononuclear cells (PBMCs) primarily produce IFN-γ. In this protocol, we describe a method for in vitro expansion and polarization of human γδT17 cells from PBMCs. Key Features • Expansion of γδ T cells from peripheral blood mononuclear cells. • Human IL-17A-producing γδ T-cell differentiation and expansion using IL-7 and anti-γδTCR. • Analysis of IL-17A production post γδ T-cell expansion.

Indexed as

IL-17Peripheral blood mononuclear cellsγδT17 cellγδ T cell

Identifiers

PMID38213324
PMCPMC10777050
OpenAlexW4390563029

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.