Evidence map›Paper›PMID 38213136›Full record

ArticleCurrent molecular medicine2025

Nrf2 Inhibits GAPDH/Siah1 Axis to Reduce Inflammatory Reactions and Proliferation of Microglia After Simulating Spinal Cord Injury.

Chunhe Sha, Feng Pan, Zhiqing Wang, Guohui Liu, Hua Wang, Tianwei Huang, Kai Huang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current molecular medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Chunhe ShaDepartment of Orthopaedics, Shanghai Jing'an District Zhabei Central Hospital, Shanghai, 200070, China.
Feng PanDepartment of Orthopaedics, Shanghai Jing'an District Zhabei Central Hospital, Shanghai, 200070, China.
Zhiqing WangDepartment of Orthopaedics, Shanghai Jing'an District Zhabei Central Hospital, Shanghai, 200070, China.
Guohui LiuDepartment of Orthopaedics, Shanghai Jing'an District Zhabei Central Hospital, Shanghai, 200070, China.
Hua WangDepartment of Orthopaedics, Shanghai Jing'an District Zhabei Central Hospital, Shanghai, 200070, China.
Tianwei HuangDepartment of Orthopaedics, Shanghai Jing'an District Zhabei Central Hospital, Shanghai, 200070, China.
Kai HuangDepartment of Orthopaedics, Shanghai Jing'an District Zhabei Central Hospital, Shanghai, 200070, China.
Shanghai Zhabei District Shibei Hospital · CN

Funding

Jing'an District Discipline Construction Project - Key Disciplines 2021ZD02
6 · The paper itself

Abstract

objectiveTo explore the effect of nuclear factor erythroid 2-related factor 2 (Nrf 2) on microglial inflammatory response and proliferation after spinal cord injury (SCI) through the glyceraldehyde phosphate dehydrogenase (GAPDH) / Seven in absentia homolog 1 (Siah 1) signaling pathway.

methodsHuman microglia HMC3 was induced by lipopolysaccharide (LPS) to establish a SCI cell model. Microglia morphology after LPS stimulation was observed by transmission electron microscope (TEM), and cellular Nrf2, GAPDH/Siah1 pathway expression and cell viability were determined. Subsequently, the Nrf2 overexpression plasmid was transfected into microglia to observe changes in cell viability and GAPDH/Siah1 pathway expression.

resultsMicroglia, mostly amoeba-like, were found to have enlarged cell bodies after LPS stimulation, with an increased number of cell branches, highly expressed Nrf2, GAPDH and Siah1, and decreased cell viability (P<0.05). Up-regulating Nrf2 inhibited the GAPDH/Siah1 axis, decreased inflammatory responses, and enhanced activity in post-SCI microglia (P<0.05).

conclusionUp-regulating Nrf2 expression can reverse the inflammatory reaction of microglia after LPS stimulation and enhance their activity by inhibiting the GAPDH/ Siah1 axis.

Indexed as

Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)InflammationMicrogliaNF-E2-Related Factor 2Spinal Cord InjuriesUbiquitin-Protein LigasesCell LineCell ProliferationCell SurvivalHumansLipopolysaccharidesSeven in Absentia ProteinsSignal TransductionGAPDH protein, humanGlyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)LipopolysaccharidesNFE2L2 protein, humanNF-E2-Related Factor 2Seven in Absentia ProteinsUbiquitin-Protein LigasesGAPDH/Siah1inflammatory reactions.microgliaNrf2signaling pathwaysspinal cord injury

Identifiers

PMID38213136
PMCPMC12272065
OpenAlexW4390793259

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.