ArticlePlant biotechnology journal2024
Editing of ORF138 restores fertility of Ogura cytoplasmic male sterile broccoli via mitoTALENs.
Article in Plant biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Establishment of an efficient and PAM-relaxed LbCas12a genome editing tool in plants.The New phytologist · 2026Article
- Mitochondrial gene orf224 triggers retrograde signaling and reprogramming of anther cell fate in Brassica napus Polima CMS.The New phytologist · 2026Article
- Harnessing mitochondrial genome editing for crop improvement: principles and applications.aBIOTECH · 2026Review
- 3-Hydroxypropanoic acid contributing to pollen abortion in ogura cytoplasmic male sterility of Brassica napus.BMC plant biology · 2026Article
- Chloroplast- and mitochondrion-specific random C-to-T mutagenesis for forward genetics of organelle genomes.The Plant journal : for cell and molecular biology · 2026Article
- Energy Metabolism and Auxin Signaling Disruption Underlying Stamen Identity Defects in Tobacco Cytoplasmic Male Sterility K326 (CMS K326): Integrated Transcriptomic and Metabolomic Analyses.Plants (Basel, Switzerland) · 2026Article
- Advancements in genomic crop techniques and considerations for regulation and food safety.Transgenic research · 2025Review
- Advances in Cytoplasmic Male Sterility in Sugar Beet from Mitochondrial Genome Structural Dynamics and Nuclear-Cytoplasmic Coordination.International journal of molecular sciences · 2025Review
- Functional relocation of the maize chloroplast atpB gene to the nucleus restores photosynthetic competence to a gene-edited non-photosynthetic mutant.Plant biotechnology journal · 2025Article
- Genome modification in plant mitochondria.Plant physiology · 2025Review
- Mitochondrial gene editing and allotopic expression unveil the role of orf125 in the induction of male fertility in some Solanum spp. hybrids and in the evolution of the common potato.Plant biotechnology journal · 2025Article
- PMGA: A plant mitochondrial genome annotator.Plant communications · 2025Article
- Past and future of cytoplasmic male sterility and heterosis breeding in crop plants.Plant cell reports · 2025Review
- Analysis ofInternational journal of molecular sciences · 2024Article
- Cytological and Molecular Characterization of a New Ogura Cytoplasmic Male Sterility Restorer ofPlants (Basel, Switzerland) · 2024Article
- Knocking out of the Mitochondrial Gene orf218 Using mitoTALECD Restores Cytoplasmic Male Sterility in Eggplant.Physiologia plantarumArticle
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
Abstract
Cytoplasmic male sterility (CMS), encoded by the mitochondrial open reading frames (ORFs), has long been used to economically produce crop hybrids. However, the utilization of CMS also hinders the exploitation of sterility and fertility variation in the absence of a restorer line, which in turn narrows the genetic background and reduces biodiversity. Here, we used a mitochondrial targeted transcription activator-like effector nuclease (mitoTALENs) to knock out ORF138 from the Ogura CMS broccoli hybrid. The knockout was confirmed by the amplification and re-sequencing read mapping to the mitochondrial genome. As a result, knockout of ORF138 restored the fertility of the CMS hybrid, and simultaneously manifested a cold-sensitive male sterility. ORF138 depletion is stably inherited to the next generation, allowing for direct use in the breeding process. In addition, we proposed a highly reliable and cost-effective toolkit to accelerate the life cycle of fertile lines from CMS-derived broccoli hybrids. By applying the k-mean clustering and interaction network analysis, we identified the central gene networks involved in the fertility restoration and cold-sensitive male sterility. Our study enables mitochondrial genome editing via mitoTALENs in Brassicaceae vegetable crops and provides evidence that the sex production machinery and its temperature-responsive ability are regulated by the mitochondria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.