Evidence map›Paper›PMID 38212867›Full record

ArticleBMC research notes2024

Simultaneous assessment of mitochondrial DNA copy number and nuclear epigenetic age towards predictive models of development and aging.

Phyo W Win, Julia Nguyen, Amanda L Morin, Charles E Newcomb, Shiva M Singh, Noha Gomaa, Christina A Castellani

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Phyo W WinDepartment of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, Canada.
Julia NguyenDepartment of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, Canada.
Amanda L MorinDepartment of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, Canada.
Charles E NewcombDepartment of Genetic Medicine, McKusick-Nathans Institute, Johns Hopkins University School of Medicine, Baltimore, USA.
Shiva M SinghDepartment of Biology, Western University, London, Canada.
Noha GomaaDepartment of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, Canada.
Christina A CastellaniDepartment of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, Canada. christina.castellani@schulich.uwo.ca.
Western University · CAChildren’s Health Research Institute · CAJohns Hopkins Medicine · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveMitochondrial dysfunction and nuclear epigenetic alterations, two hallmarks of aging, are associated with aberrant development and complex disease risk. Here, we report a method for the simultaneous assessment of mitochondrial DNA copy number (mtDNA-CN) and DNA methylation age (DNAm age) from the same DNA extraction using quantitative polymerase chain reaction (qPCR) and array data, respectively.

resultWe present methods for the concurrent estimation of mtDNA-CN and DNAm age from the same DNA samples. This includes qPCR to estimate mtDNA-CN, representing the number of circular mitochondrial genomes in a cell, and DNA methylation microarray data to estimate the epigenetic age of an individual. Further, we provide a method for the combination of these metrics into a shared metric termed 'mtEpiAge'. This approach provides a valuable tool for exploring the interplay between mitochondrial dysfunction and nuclear epigenetic alterations, and their associations with disease and aging.

Indexed as

DNA, MitochondrialMitochondrial DiseasesAgingDNA Copy Number VariationsEpigenesis, GeneticHumansDNA, MitochondrialDNA methylationEpigenetic ageMitochondrial DNAMitochondrial DNA copy numberqPCR

Identifiers

PMID38212867
PMCPMC10785513
OpenAlexW4390738853

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.