ArticleNature neuroscience2024
Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses.
Article in Nature neuroscience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Mapping brain-wide monosynaptic inputs to single neurons with ROInet-seq.Cell reports methods · 2026Article
- An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish.eLife · 2026Article
- TRACR: an anterograde transneuronal tracing system for genetic access across synapses and longitudinal circuit analysis.bioRxiv : the preprint server for biology · 2026Article
- Postoperative Impairment of GluA2 Ttrafficking in CA3 Induces Memory Consolidation Deficits in Aged Mice by Disrupting SPW-R in CA1.Journal of molecular neuroscience : MN · 2026Article
- Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas.Nature protocols · 2026Review
- Transsynaptic viral tools in neural circuit analysis: from anatomical mapping to functional interrogation.Frontiers in neural circuits · 2026Review
- Dissecting mammalian cortical circuit development at single-cell resolution using inducible barcoded rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Circuit-specific gene editing for precision modulation of neuronal activity with CRISPR-rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Hippocampal neural circuit mechanisms in Alzheimer's disease revealed by viral-genetic circuit mapping.Neurobiology of disease · 2025Review
- A scalable, all-optical method for mapping synaptic connectivity with cell-type specificity.bioRxiv : the preprint server for biology · 2025Article
- Development of Cre-dependent retrograde trans-multisynaptic tracer based on pseudorabies virus bartha strain.Molecular brain · 2025Article
- Molecular Engineering of Virus Tropism.International journal of molecular sciences · 2024Review
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing.eLife · 2024Article
- Third-generation rabies viral vectors have low toxicity and improved efficiency as retrograde labeling tools.Cell reports methods · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
19 authors at 6 institutions in 2 countries.
Funding
Abstract
Rabies-virus-based monosynaptic tracing is a widely used technique for mapping neural circuitry, but its cytotoxicity has confined it primarily to anatomical applications. Here we present a second-generation system for labeling direct inputs to targeted neuronal populations with minimal toxicity, using double-deletion-mutant rabies viruses. Viral spread requires expression of both deleted viral genes in trans in postsynaptic source cells. Suppressing this expression with doxycycline following an initial period of viral replication reduces toxicity to postsynaptic cells. Longitudinal two-photon imaging in vivo indicated that over 90% of both presynaptic and source cells survived for the full 12-week course of imaging. Ex vivo whole-cell recordings at 5 weeks postinfection showed that the second-generation system perturbs input and source cells much less than the first-generation system. Finally, two-photon calcium imaging of labeled networks of visual cortex neurons showed that their visual response properties appeared normal for 10 weeks, the longest we followed them.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.