Evidence map›Paper›PMID 38206205›Full record

ArticleHepatology communications2024

Myeloid spatial and transcriptional molecular signature of ischemia-reperfusion injury in human liver transplantation.

Rebecca A Sosa, Richard Ahn, Fang Li, Allyson Q Terry, Zach Qian, Adil Bhat, Subha Sen, Bita V Naini, Takahiro Ito, Fady M Kaldas and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Hepatology communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 1 institution in 1 country.

Rebecca A SosaDepertment of Pathology and Laboratory Medicine, UCLA, Los Angeles, California, USA.
Richard AhnInstitute for Quantitative and Computational Biosciences, UCLA, Los Angeles, California, USA.
Fang LiDepertment of Pathology and Laboratory Medicine, UCLA, Los Angeles, California, USA.
Allyson Q TerryDepertment of Pathology and Laboratory Medicine, UCLA, Los Angeles, California, USA.
Zach QianInstitute for Quantitative and Computational Biosciences, UCLA, Los Angeles, California, USA.
Adil BhatDepertment of Pathology and Laboratory Medicine, UCLA, Los Angeles, California, USA.
Subha SenDepertment of Pathology and Laboratory Medicine, UCLA, Los Angeles, California, USA.
Bita V NainiDepertment of Pathology and Laboratory Medicine, UCLA, Los Angeles, California, USA.
Takahiro ItoDepertment of Surgery, UCLA, Los Angeles, California, USA.
Fady M KaldasDepertment of Surgery, UCLA, Los Angeles, California, USA.
Alexander HoffmannInstitute for Quantitative and Computational Biosciences, UCLA, Los Angeles, California, USA.ORCID 0000-0002-9860-6830
Ronald W BusuttilDepertment of Surgery, UCLA, Los Angeles, California, USA.
Jerzy W Kupiec-WeglinskiDepertment of Pathology and Laboratory Medicine, UCLA, Los Angeles, California, USA.
David W GjertsonDepertment of Pathology and Laboratory Medicine, UCLA, Los Angeles, California, USA.
Elaine F ReedDepertment of Pathology and Laboratory Medicine, UCLA, Los Angeles, California, USA.
University of California, Los Angeles · US

Funding

UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
Regulation of Innate Immune Responses by Alloimmunity in Liver Ischemia-Reperfusion InjuryP01AI120944 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Fady M Kaldas · 2017 to 2026
$20.0M
UCLA Tumor Immunology Training ProgramT32CA009120 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Steven M. Dubinett, MICHAEL A TEITELL · 1985 to 2026
$10.3M
NCATS NIH HHS UL1 TR001881NCI NIH HHS T32 CA009120NIAID NIH HHS P01 AI120944NIMHD NIH HHS L60 MD011903
6 · The paper itself

Abstract

backgroundIschemia-reperfusion injury (IRI) is a significant clinical concern in liver transplantation, with a key influence on short-term and long-term allograft and patient survival. Myeloid cells trigger and sustain tissue inflammation and damage associated with IRI, but the mechanisms regulating these activities are unknown. To address this, we investigated the molecular characteristics of intragraft myeloid cells present in biopsy-proven IRI- and IRI+ liver transplants.

methodsRNA-sequencing was performed on 80 pre-reperfusion and post-reperfusion biopsies from 40 human recipients of liver transplantation (23 IRI+, 17 IRI-). We used transcriptional profiling and computational approaches to identify specific gene coexpression network modules correlated with functional subsets of MPO+, lysozyme+, and CD68+ myeloid cells quantified by immunohistochemistry on sequential sections from the same patient biopsies.

resultsA global molecular map showed gene signatures related to myeloid activation in all patients regardless of IRI status; however, myeloid cell subsets differed dramatically in their spatial morphology and associated gene signatures. IRI- recipients were found to have a natural corticosteroid production and response profile from pre-reperfusion to post-reperfusion, particularly among monocytes/macrophages. The pre-reperfusion signature of IRI+ recipients included acute inflammatory responses in neutrophils and increased translation of adaptive immune-related genes in monocytes/macrophages coupled with decreased glucocorticoid responses. Subsequent lymphocyte activation at post-reperfusion identified transcriptional programs associated with the transition to adaptive immunity found only among IRI+ recipients.

conclusionsMyeloid subset-specific genes and related signaling pathways provide targets for the development of therapeutic strategies aimed at limiting IRI in the clinical setting of liver transplantation.

Indexed as

Liver TransplantationReperfusion InjuryAdaptive ImmunityBiopsyHumansInflammationLeukocytes

Identifiers

PMID38206205
PMCPMC10786592
OpenAlexW4390753701

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.