Increasing the Activity of the High-Fidelity SpyCas9 Form in Yeast by Directed Mutagenesis of the PAM-Interacting Domain.

Artem I Davletshin, Anna A Matveeva, Stanislav S Bachurin, Dmitry S Karpov, David G Garbuz

Open access · goldAbstract read

In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 39% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Engineering Cas9: next generation of genomic editors.Applied microbiology and biotechnology · 2024
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Artem I DavletshinEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.ORCID 0000-0002-2635-9279

Anna A MatveevaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.

Stanislav S BachurinFSBEI HE Rostov State Medical University, Ministry of Health, 344022 Rostov-on-Don, Russia.

Dmitry S KarpovEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.ORCID 0000-0001-5203-0787

David G GarbuzEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.

Engelhardt Institute of Molecular Biology · RURostov State Medical University · RU

Funding

Ministry of Science and Higher Education of the Russian Federation 075-15-2019-1660Russian Science Foundation 22-14-00377

6 · The paper itself

Abstract

CRISPR/Cas systems are used for genome editing, both in basic science and in biotechnology. However, CRISPR/Cas editors have several limitations, including insufficient specificity leading to "off-targets" and the dependence of activity on chromatin state. A number of highly specific Cas9 variants have now been obtained, but most of them are characterized by reduced activity on eukaryotic chromatin. We identified a spatial cluster of amino acid residues in the PAM-recognizing domain of

Indexed as

ChromatinSaccharomyces cerevisiaeAmino AcidsMutagenesisMutationAmino AcidsChromatinCRISPR/Cas9genome editinghigh-fidelity SpyCas9 formsSaccharomyces cerevisiaesite-directed mutagenesis

Identifiers

PMID38203615

DOI10.3390/ijms25010444

PMCPMC10779060

OpenAlexW4390338768

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.