Evidence map›Paper›PMID 38203197›Full record

ReviewInternational journal of molecular sciences2023

Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy.

Sejoo Jeong, Dongbin Koh, Eunha Gwak, Chinmaya V Srambickal, Daeha Seo, Jerker Widengren, Jong-Chan Lee

Abstract readReview
In one paragraph

Review in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sejoo JeongDepartment of New Biology, DGIST, Daegu 42988, Republic of Korea.
Dongbin KohSchool of Undergraduate Studies, DGIST, Daegu 42988, Republic of Korea.
Eunha GwakDepartment of New Biology, DGIST, Daegu 42988, Republic of Korea.
Chinmaya V SrambickalExp. Biomol. Physics, Dept. Applied Physics, KTH-Royal Institute of Technology, 106 91 Stockholm, Sweden.
Daeha SeoDepartment of Physics and Chemistry, DGIST, Daegu 42988, Republic of Korea.
Jerker WidengrenExp. Biomol. Physics, Dept. Applied Physics, KTH-Royal Institute of Technology, 106 91 Stockholm, Sweden.ORCID 0000-0003-3200-0374
Jong-Chan LeeDepartment of New Biology, DGIST, Daegu 42988, Republic of Korea.ORCID 0000-0003-3073-4657

Funding

DGIST R&D Program of the Ministry of Science and ICT of Korea 20-CoE-BT-04National Research Foundation of Korea NRF-2019K2A9A2A12000363National Research Foundation of Korea NRF-2021R1I1A2058828National Research Foundation of Korea RS-2023-00252979Swedish Cancer Society 201248 PjF01HSwedish Foundation for International Cooperation in Research and Higher Education KO2018-7991
6 · The paper itself

Abstract

Optical nanoscopy, also known as super-resolution optical microscopy, has provided scientists with the means to surpass the diffraction limit of light microscopy and attain new insights into nanoscopic structures and processes that were previously inaccessible. In recent decades, numerous studies have endeavored to enhance super-resolution microscopy in terms of its spatial (lateral) resolution, axial resolution, and temporal resolution. In this review, we discuss recent efforts to push the resolution limit of stimulated emission depletion (STED) optical nanoscopy across multiple dimensions, including lateral resolution, axial resolution, temporal resolution, and labeling precision. We introduce promising techniques and methodologies building on the STED concept that have emerged in the field, such as MINSTED, isotropic STED, and event-triggered STED, and evaluate their respective strengths and limitations. Moreover, we discuss trade-off relationships that exist in far-field optical microscopy and how they come about in STED optical nanoscopy. By examining the latest developments addressing these aspects, we aim to provide an updated overview of the current state of STED nanoscopy and its potential for future research.

Indexed as

Microscopyadaptive illuminationfluorescence lifetimefluorescent proteinfluorophore localizationnanobodyoptical nanoscopyresolutionSTEDsuper-resolution microscopytemporal resolution

Identifiers

PMID38203197
PMCPMC10779414

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.