Evidence map›Paper›PMID 38200552›Full record

ArticleBMC veterinary research2024

Acidification is required for calcium and magnesium concentration measurements in equine urine.

Sandra Lapsina, Martina Stirn, Regina Hofmann-Lehmann, Angelika Schoster, Barbara Riond

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Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Sandra LapsinaClinical Laboratory, Department for Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Martina StirnClinical Laboratory, Department for Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Regina Hofmann-LehmannClinical Laboratory, Department for Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Angelika SchosterClinic for Equine Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Barbara RiondClinical Laboratory, Department for Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland. briond@vetclinics.uzh.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAcidification of equine urine to promote dissociation of ion complexes is a common practice for urine ion concentration measurements. The objective of this study was to evaluate the effect of acidification and storage after acidification on calcium (Ca), magnesium (Mg) and phosphate (P) concentrations and on fractional excretion (FE) of these electrolytes. Thirty-two fresh equine urine samples were analysed between December 2016 and July 2020. Complete urinalysis (stick and sediment) was performed on all samples. Ca, Mg, P and creatinine concentrations were measured in supernatant of centrifuged native urine, urine directly centrifuged after acidification and urine centrifuged 1 hour after acidification. Urine was acidified with hydrochloric acid to reach a pH of 1-2. Ca, Mg, P and creatinine concentrations were also measured in blood plasma, and fractional excretion of each electrolyte was calculated. Equality of medians was tested with Friedman tests and Bland-Altman bias plots were used to show the agreement between conditions.

resultsAcidification had a statistically significant effect on Ca and Mg concentrations, FE

conclusionsUrine acidification is essential for accurate measurement of Ca and Mg concentrations and therefore FE calculations in equine urine. Storage time of 1 hour after acidification does not significantly change Ca and Mg concentrations.

Indexed as

Body FluidsCalciumAnimalsCreatinineHorsesHydrogen-Ion ConcentrationMagnesiumCalciumCreatinineMagnesiumFractional excretion of calcium and magnesiumHydrochloric acidPhosphate

Identifiers

PMID38200552
PMCPMC10777620

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