Evidence map›Paper›PMID 38198069›Full record

ArticleVirus genes2024

DsRNA sequencing revealed a previously missed terminal sequence of a +ssRNA virus that infects dinoflagellate Heterocapsa circularisquama.

Michiko Takahashi, Yuichi Masuda, Yuto Chiba, Syun-Ichi Urayama, Keizo Nagasaki

Abstract read
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In one paragraph

Article in Virus genes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 100% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Michiko TakahashiKochi Medical School, Kochi University, Nankoku, Kochi, 783-8505, Japan.
Yuichi MasudaFaculty of Agriculture and Marine Science, Kochi University, Nankoku, Kochi, 783-8502, Japan.
Yuto ChibaLaboratory of Fungal Interaction and Molecular Biology (Donated by IFO), Department of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Syun-Ichi UrayamaLaboratory of Fungal Interaction and Molecular Biology (Donated by IFO), Department of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Keizo NagasakiFaculty of Agriculture and Marine Science, Kochi University, Nankoku, Kochi, 783-8502, Japan. nagasaki@kochi-u.ac.jp.
Kōchi University · JPUniversity of Tsukuba · JP

Funding

ACT-X, Japan Science and Technology Agency JPMJAX21BDJapan Society for the Promotion of Science JP19J00346Ministry of Education, Culture, Sports, Science and Technology of Japan JP16H06429Ministry of Education, Culture, Sports, Science and Technology of Japan JP16H06437Ministry of Education, Culture, Sports, Science and Technology of Japan JP16K21723
6 · The paper itself

Abstract

Heterocapsa circularisquama RNA virus (HcRNAV) is the only dinoflagellate-infecting RNA virus cultured. However, only two strains of HcRNAV have been registered with complete genome sequences (strains 34 and 109 for UA and CY types, respectively). To extend the genomic information of HcRNAV, we performed full-genome sequencing of an unsequenced strain of HcRNAV (strain A8) using the fragmented and primer-ligated double-stranded RNA (dsRNA) sequencing (FLDS) method. The complete genome of HcRNAV A8 with 4457 nucleotides (nt) was successfully determined, and sequence alignment of the major capsid protein gene suggested that A8 was a UA-type strain, consistent with its intraspecific host specificity. The complete sequence was found to be 80 nt longer at the 5' terminus than the registered sequences of HcRNAV strains (34 and 109), suggesting that FLDS is more reliable for determining the terminal sequence than conventional methods (5' Rapid Amplification of cDNA End). Our study contributes to a better understanding of dinoflagellate-infecting viruses with limited sequence data.

Indexed as

DinoflagellidaRNA VirusesVirusesRNA, Double-StrandedRNA, ViralSequence AlignmentRNA, Double-StrandedRNA, ViralComplete genomeDinoflagellateHeterocapsa circularisquama RNA virus (HcRNAV)Red tide

Identifiers

PMID38198069
OpenAlexW4390702003

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.