Evidence map›Paper›PMID 38197255›Full record

ArticleThe Annals of otology, rhinology, and laryngology2024

Dynamic Molecular Markers of Otosclerosis in the Human Cochlea.

Sarah Hodge, Ivan A Lopez, Alex Cronkite, John House, Hirooki Matsui, Gail Ishiyama, Akira Ishiyama

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Annals of otology, rhinology, and laryngology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.2field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Sarah HodgeDepartment of Otolaryngology-Head and Neck Surgery, Augusta University/Medical College of Georgia, Augusta, GA, USA.ORCID 0000-0001-5113-3864
Ivan A LopezDepartment of Otolaryngology-Head and Neck Surgery, University of California Los Angeles, Los Angeles, CA, USA.ORCID 0000-0001-5205-7293
Alex CronkiteDepartment of Otolaryngology-Head and Neck Surgery, University of California Los Angeles, Los Angeles, CA, USA.
John HouseHouse Ear Clinic, Los Angeles, CA, USA.
Hirooki MatsuiYamagata University School of Medicine, Yamagata, Japan.
Gail IshiyamaDepartment of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
Akira IshiyamaDepartment of Otolaryngology-Head and Neck Surgery, University of California Los Angeles, Los Angeles, CA, USA.
University of California, Los Angeles · USAugusta University · USHouse Clinic · USYamagata University · JP

Funding

The Human Temporal Bone Laboratory Resource for Basic and Applied Inner Ear SciencesU24DC015910 · NIDCD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ISHIYAMA, AKIRA · 2017 to 2022
$4.2M
Modern Cellular and Molecular Techniques to Study the Human Temporal BoneU24DC020855 · NIDCD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Akira Ishiyama · 2023 to 2026
$2.7M
NIDCD NIH HHS U24 DC015910NIDCD NIH HHS U24 DC020855
6 · The paper itself

Abstract

objectiveTo investigate the role and distribution of various molecular markers using immunohistochemistry and immunofluorescence to further elucidate and understand the pathogenesis of otosclerosis.

methodsArchival celloidin formalin-fixed 20-micron thick histologic sections from 7 patients diagnosed with otosclerosis were studied and compared to controls. Sections in the mid-modiolar region were immunoreacted with rabbit polyclonal antibodies against nidogen-1, β2-laminin, collagen-IX, BSP, and monoclonal antibodies against TGF β-1 and ubiquitin. Digital images were acquired using a high-resolution light and laser confocal microscope.

resultsNidogen-1, BSP, and collagen-IX were expressed in the otospongiotic regions, and to lesser extent, in the otosclerotic regions, the latter previously believed to be inactive. β2-laminin and ubiquitin were uniformly expressed in both otospongiotic and otosclerotic regions. There was a basal level of expression of all of these markers in the normal hearing and sensorineural hearing loss specimens utilized as control. TGF β -1, however, though present in the otosclerosis bones, was absent in the normal hearing and sensorineural hearing loss controls.

conclusionsOur results propose that the activity and function of TGF-1 may play a key role in the development and pathogenesis of otosclerosis. Further studies utilizing a higher number of temporal bone specimens will be helpful for future analysis and to help decipher its role as a potential target in therapeutic interventions.

Indexed as

Hearing Loss, SensorineuralOtosclerosisAnimalsCochleaCollagenHumansLamininRabbitsUbiquitinsCollagenLamininUbiquitinsimmunofluorescenceimmunohistochemistryotosclerosis

Identifiers

PMID38197255
PMCPMC11057044
OpenAlexW4390701750

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.