Evidence map›Paper›PMID 38195704›Full record

ArticleScientific reports2024

Establishment of a forward primers-superposed amplification analysis for accurate aspirin pharmacogenomic measurement.

Chun-Yan Li, Ping Yang, Jie Zheng, Jing Zhang, Yi-Qing Liu, Xiao-Quan Liu, Yue Hu, Wen-Jun Lan

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Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Chun-Yan Li *Institute of Biomedical Engineering, Qilu University of Technology (Shandong Academy of Sciences), No.3501, Daxue Road, Jinan, 250353, China.
Ping Yang *Institute of Biomedical Engineering, Qilu University of Technology (Shandong Academy of Sciences), No.3501, Daxue Road, Jinan, 250353, China.
Jie ZhengInstitute of Biomedical Engineering, Qilu University of Technology (Shandong Academy of Sciences), No.3501, Daxue Road, Jinan, 250353, China.
Jing ZhangInstitute of Biomedical Engineering, Qilu University of Technology (Shandong Academy of Sciences), No.3501, Daxue Road, Jinan, 250353, China.
Yi-Qing LiuDepartment of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250000, China.
Xiao-Quan LiuInstitute of Biomedical Engineering, Qilu University of Technology (Shandong Academy of Sciences), No.3501, Daxue Road, Jinan, 250353, China.
Yue HuInstitute of Biomedical Engineering, Qilu University of Technology (Shandong Academy of Sciences), No.3501, Daxue Road, Jinan, 250353, China. huyue@qlu.edu.cn.
Wen-Jun LanInstitute of Biomedical Engineering, Qilu University of Technology (Shandong Academy of Sciences), No.3501, Daxue Road, Jinan, 250353, China. lanwenjun0522@qlu.edu.cn.

Funding

The key project of international cooperation of Qilu University of Technology No. QLUTGJHZ2018008
6 · The paper itself

Abstract

Genotyping of gDNA rs12041331 (PEAR1), rs6065 (GP1BA), and rs730012 (LTC4S) can provide systematic guidance on the use of aspirin. However, an accurate, reliable and economical approach to simultaneous detection of the above single nucleotide polymorphisms (SNPs) is not reported. Herein, we designed and substantiated an allele-specific (AS) forward primer-superposed amplification analysis for measurement of the SNPs in PEAR1, GP1BA and LTC4S genes, in which the values of ∆Cq (differences in threshold cycles between the wild-type forward primer-based assay and the mutated-type forward primer-based assay) were employed to decide genotype. Mismatch AS forward primers were screened with the singleplex amplification analysis. Moreover, Cq extension optimized by AS forward primer superposition was observed in the selected forward primer-based triplex analysis. Further, robustness assessment of the triplex analysis showed the amplification efficiency ranging from 0.9 to 1.1. Precision test demonstrated the coefficient of variation of less than 2%. And the detective results of 189 DNA samples was completely concordant with that of commercial Sanger sequencing. In summary, we developed a simple, accurate and economical approach to genotyping of rs12041331 (PEAR1), rs6065 (GP1BA) and rs730012 (LTC4S) to provide a valuable pharmacogenomics tool for guidance of aspirin delivery.

Indexed as

AspirinPharmacogeneticsAllelesBiological AssayGenotypeAspirin

Identifiers

PMID38195704
PMCPMC10776573

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.