Sample preparation conditions for the real-time measurement of W/O emulsions by resonance-enhanced multiphoton ionization time-of-flight mass spectrometry.

Analysis of an emulsion in its original dispersed condition is quite important for quality assessment and quality control. In the present study, the practical experimental conditions of the real-time measurement of a water-in-oil (W/O) emulsion were examined via resonance-enhanced multiphoton ionization time-of-flight mass spectrometry (REMPI-TOFMS). A W/O emulsion was prepared using cyclohexane as the oil phase with toluene as an analyte species. A time profile of the peak area for toluene was constructed based on the mass spectra. Normally, the negative spikes of a base signal are detected in a time profile when analyte molecules are dispersed in an oil phase. In this case, however, the positive spikes were unexpectedly detected rather than the negative ones. Though several factors could be relevant for the occurrence of the positive spikes, these spikes could have been suppressed by the addition of a small amount of n-alkane when the oil phase was prepared in the present study. The practical experimental conditions for the analysis of a W/O emulsion in real-time revealed that this method would be applicable to the analysis of an oil-in-water-in-oil (O/W/O) emulsion where the outer phase is also an oil phase.