ArticleNature methods2024
Mapping enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons.
Article in Nature methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Achieving cell-type-specific bioorthogonal chemistry using enzyme-activated caged tetrazines.Nature chemical biology · 2026Article
- FILM: mapping organellar metabolism by mid-infrared photothermal-modulated fluorescence.Nature methods · 2026Article
- FILM: Mapping organellar metabolism by mid-infrared photothermal modulated fluorescence.ArXiv · 2026Article
- Far-field single-molecule vibrational spectroscopy and imaging.Chemical science · 2026Review
- Bond-Selective Imaging at the Frontier of Biomedicine.Chemical & biomedical imaging · 2025Article
- Feeling the vibes: Vibrational spectroscopic imaging of biomolecular assemblies in their natural environment.Applied physics reviews · 2025Article
- Functional Raman Probes for Detecting Enzyme Activities Based on Aggregation Control.Analytical chemistry · 2025Article
- Self-Supervised Elimination of Non-Independent Noise in Hyperspectral Imaging.Newton ((New York, N.Y.) · 2025Article
- Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy.Nature communications · 2025Article
- A 20-year journey on the invention of vibrational photothermal microscopy.Nature methods · 2025Article
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Background-Free Mid-Infrared Photothermal Microscopy via Single-Shot Measurement of Thermal Decay.Analytical chemistry · 2025Article
- Room-Temperature Single-Molecule Infrared Imaging and Spectroscopy through Bond-Selective Fluorescence.Angewandte Chemie (International ed. in English) · 2024Article
- Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe.Science advances · 2024Article
- Smart Molecular Imaging and Theranostic Probes by Enzymatic Molecular In Situ Self-Assembly.JACS Au · 2024Review
- Overtone photothermal microscopy for high-resolution and high-sensitivity vibrational imaging.Nature communications · 2024Article
- Type-printable photodetector arrays for multichannel meta-infrared imaging.Nature communications · 2024Article
- Mid-infrared Photothermal Imaging: Instrument and Life Science Applications.Analytical chemistry · 2024Review
- Enzymatic control of intermolecular interactions for generating synthetic nanoarchitectures in cellular environment.Science and technology of advanced materials · 2024Review
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11 authors at 2 institutions in 1 country.
Funding
Abstract
Simultaneous spatial mapping of the activity of multiple enzymes in a living system can elucidate their functions in health and disease. However, methods based on monitoring fluorescent substrates are limited. Here, we report the development of nitrile (C≡N)-tagged enzyme activity reporters, named nitrile chameleons, for the peak shift between substrate and product. To image these reporters in real time, we developed a laser-scanning mid-infrared photothermal imaging system capable of imaging the enzymatic substrates and products at a resolution of 300 nm. We show that when combined, these tools can map the activity distribution of different enzymes and measure their relative catalytic efficiency in living systems such as cancer cells, Caenorhabditis elegans, and brain tissues, and can be used to directly visualize caspase-phosphatase interactions during apoptosis. Our method is generally applicable to a broad category of enzymes and will enable new analyses of enzymes in their native context.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.