ArticleNature communications2024
Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Mapping the Cerebral Organoid Landscape: A Systematic Review of Preclinical 3D Models in Neuroscience.Advanced healthcare materials · 2026Pooled it
- Demystifying Experimental Parameters in Optical Photothermal Imaging in Aqueous Media.ACS omega · 2026Article
- Optical photothermal infrared imaging of fatty acid esterification in the ER of living cells.Science advances · 2026Article
- Programmable Construction of Supramolecular Polymers Achieved in Neutral Lipid Environments.Nature communications · 2026Article
- Illuminating aging with multimodal optical metabolic imaging.Science advances · 2026Review
- Biological and Biomedical Applications of Optical Photothermal Infrared Spectroscopy (O-PTIR).Applied spectroscopy · 2026Review
- Optical photothermal infrared imaging of fatty acid esterification in the ER of living cells.bioRxiv : the preprint server for biology · 2026Article
- Bond-Selective Imaging at the Frontier of Biomedicine.Chemical & biomedical imaging · 2025Article
- Feeling the vibes: Vibrational spectroscopic imaging of biomolecular assemblies in their natural environment.Applied physics reviews · 2025Article
- "Golden cicada-escape" style colon-targeted pellets for ulcerative colitis by balancing oxidative stress and repairing colonic barrier.Materials today. Bio · 2025Article
- Cholesterol modulates membrane elasticity via unified biophysical laws.Nature communications · 2025Article
- Combining Submicron Spectroscopy Techniques (AFM-IR and O-PTIR) To Detect and Quantify Microplastics and Nanoplastics in Snow from a Utah Ski Resort.Environmental science & technology · 2025Article
- COAdvanced materials (Deerfield Beach, Fla.) · 2025Article
- Deep MALDI-MS spatial omics guided by quantum cascade laser mid-infrared imaging microscopy.Nature communications · 2025Article
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Review
- Modelling human brain development and disease with organoids.Nature reviews. Molecular cell biology · 2025Review
- Optical Photothermal Infrared Imaging Using Metabolic Probes in Biological Systems.Analytical chemistry · 2025Article
- Progranulin deficiency in the brain: the interplay between neuronal and non-neuronal cells.Translational neurodegeneration · 2025Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Understanding metabolic heterogeneity is the key to uncovering the underlying mechanisms of metabolic-related diseases. Current metabolic imaging studies suffer from limitations including low resolution and specificity, and the model systems utilized often lack human relevance. Here, we present a single-cell metabolic imaging platform to enable direct imaging of lipid metabolism with high specificity in various human-derived 2D and 3D culture systems. Through the incorporation of an azide-tagged infrared probe, selective detection of newly synthesized lipids in cells and tissue became possible, while simultaneous fluorescence imaging enabled cell-type identification in complex tissues. In proof-of-concept experiments, newly synthesized lipids were directly visualized in human-relevant model systems among different cell types, mutation status, differentiation stages, and over time. We identified upregulated lipid metabolism in progranulin-knockdown human induced pluripotent stem cells and in their differentiated microglia cells. Furthermore, we observed that neurons in brain organoids exhibited a significantly lower lipid metabolism compared to astrocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.