Evidence map›Paper›PMID 38190029›Full record

ArticleJournal of endocrinological investigation2024

Role of thyroid stimulating hormone in the maintenance and functioning of the human corpus luteum.

M Taggi, C Capponi, N Bertani, G Saturno, F Innocenti, L Dovere, S M Fabozzi, O Alesiani, V Arena, D Cimadomo and 6 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of endocrinological investigation, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.4field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 1 country.

M TaggiDAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Rome, Italy.
C CapponiDAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Rome, Italy.
N BertaniDAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Rome, Italy.
G SaturnoDAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Rome, Italy.
F InnocentiIVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, Rome, Italy.
L DovereIVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, Rome, Italy.
S M FabozziIVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, Rome, Italy.
O AlesianiDepartment Tutela della Salute della Donna, del Bambino e di Sanità Pubblica, Università Cattolica del Sacro Cuore, Rome, Italy.
V ArenaDepartment of Woman and Child Health and Public Health, Area of Pathology, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.
D CimadomoIVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, Rome, Italy.
R MazzilliIVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, Rome, Italy.
L RienziIVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, Rome, Italy.
F M UbaldiIVIRMA Global Research Alliance, GENERA, Clinica Valle Giulia, Rome, Italy.
R CanipariDAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Rome, Italy. rita.canipari@fondazione.uniroma1.it.ORCID http://orcid.org/0000-0003-3481-6908
E ViciniDAHFMO, Unit of Histology and Medical Embryology, Sapienza, University of Rome, Rome, Italy. elena.vicini@uniroma1.it.
R ApaDepartment Tutela della Salute della Donna, del Bambino e di Sanità Pubblica, Università Cattolica del Sacro Cuore, Rome, Italy.
Clinica Valle Giulia · ITSapienza University of Rome · ITUniversità Cattolica del Sacro Cuore · ITAgostino Gemelli University Polyclinic · ITUniversity of Urbino · IT

Funding

Ministero dell'Istruzione, dell'Università e della Ricerca PRIN 2017 2017TK7Z8L
6 · The paper itself

Abstract

purposeTo evaluate the impact of high thyroid stimulating hormone (TSH) levels on human granulosa-luteal (hGL) cells.

methodshGL cells were isolated from follicular aspirates derived from patients undergoing IVF treatment without any thyroid disorder (serum TSH 0.5-2 mU/L). Cells were cultured at 37 °C in DMEM, supplemented with 5% FBS. The cells were treated with 1 nM LH and increasing concentrations of TSH. At the end of culture, conditioned medium and cells were collected to analyze progesterone production, cell viability, and mRNA levels of genes involved in the steroidogenesis process. Human ovarian tissues were analyzed for TSH receptor (TSHR) expression by IHC.

resultsThe expression of TSHR was detected in human corpus luteum by IHC and in hGL by RT-PCR. In hGL cells, TSH treatment did not modulate progesterone production nor the expression of steroidogenic genes, such as p450scc and HSD3b 1/2. However, TSH induced a dose-dependent increase in cell death. Finally, TSH did not affect LH-induced p450scc and HSD3b1/2 expression while LH partially reverted TSH negative effect on cell death in hGL.

conclusionsElevated TSH levels in hypothyroid women may be associated with impaired CL functioning and maintenance. These findings open a new line of research for the importance of the treatment of women with thyroid dysfunction that could contribute to the onset of infertility.

Indexed as

Corpus LuteumThyrotropinAdultCells, CulturedCell SurvivalFemaleHumansLuteal CellsLuteinizing HormoneProgesteroneReceptors, ThyrotropinLuteinizing HormoneProgesteroneReceptors, ThyrotropinThyrotropinGranulosa luteal cellsHuman ovaryProgesteroneTSH

Identifiers

PMID38190029
OpenAlexW4390672056

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.