ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023
[Rapamycin enhances inhibitory effect of RSL3 on proliferation, invasion and migration of testicular cancer I-10 cells
Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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5 authors at 1 institution in 1 country.
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Abstract
objectiveTo investigate the effect of rapamycin for enhancing the inhibitory effect of RSL3 on proliferation, invasion and migration of testicular cancer I-10 cells
methodsI-10 cells treated with 0-16 μmol/L RSL3 (Type Ⅱ) either alone or in combination with 16 μmol/L rapamycin (RAPA) were examined for changes in proliferation using MTT assay and colonyforming assay, and the changes in cell migration and invasion abilities were detected with wounding-healing assay and Transwell assay. The changes in the levels of lipid reactive oxygen species in the treated cells were detected using flow cytometry. GSH and MDA contents in the cells were detected using commercial detection kits, and GPX4 protein expression level was determined with Western blotting.
resultsThe cytotoxic effect of RSL3 increased dose-dependently in I-10 cells, and the combined treatment with rapamycin further enhanced its cytotoxicity. Treatment of I-10 cells with RSL3 alone significantly decreased cell colony numbers (
conclusionRapamycin enhances the inhibitory effect of RSL3 on proliferation, invasion and migration of I-10 cells by enhancing RSL3-mediated cell ferroptosis.
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