Evidence map›Paper›PMID 38187535›Full record

ArticlebioRxiv : the preprint server for biology2025

Visualizing PIEZO1 Localization and Activity in hiPSC-Derived Single Cells and Organoids with HaloTag Technology.

Gabriella A Bertaccini, Ignasi Casanellas, Elizabeth L Evans, Jamison L Nourse, George D Dickinson, Gaoxiang Liu, Sayan Seal, Alan T Ly, Jesse R Holt, Tharaka D Wijerathne and 7 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 6 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 4 institutions in 1 country.

Gabriella A BertacciniDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0002-4390-8583
Ignasi CasanellasDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0002-5264-169X
Elizabeth L EvansDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0003-1237-7262
Jamison L NourseDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0001-6240-3209
George D DickinsonDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0001-7711-0388
Gaoxiang LiuAdvanced Bioimaging Center, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID 0000-0002-1001-0064
Sayan SealAdvanced Bioimaging Center, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID 0009-0004-8897-173X
Alan T LyDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0002-9738-0200
Jesse R HoltDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0001-8136-6394
Tharaka D WijerathneDepartment of Basic Medical Sciences, Western University of Health Sciences, Pomona, CA, USA.ORCID 0000-0002-6900-3272
Shijun YanDepartment of Biomedical Engineering, University of California, Irvine, CA, USA.
Elliot E HuiDepartment of Biomedical Engineering, University of California, Irvine, CA, USA.ORCID 0000-0001-8286-9815
Jerome J LacroixDepartment of Basic Medical Sciences, Western University of Health Sciences, Pomona, CA, USA.ORCID 0000-0001-5687-0652
Mitradas M PanickerDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0002-0782-6222
Srigokul UpadhyayulaAdvanced Bioimaging Center, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID 0000-0001-6727-5883
Ian ParkerDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.
Medha M PathakDepartment of Physiology and Biophysics, University of California, Irvine, CA, USA.ORCID 0000-0002-6518-3085
University of California, Irvine · USElucid Bioimaging · USWestern University of Health Sciences · USLawrence Berkeley National Laboratory · US

Funding

Training Program in Stem Cell Translational Medicine for Neurological DisordersT32NS082174 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PETER John DONOVAN, Leslie Michels Thompson · 2013 to 2026
$3.3M
Building the brain: How mechanical forces shape human neural developmentDP2AT010376 · NCCIH · UNIVERSITY OF CALIFORNIA-IRVINE · PI PATHAK, MEDHA M · 2018 to 2020
$2.9M
Piezo1 in neural stem cell mechano-regulationR01NS109810 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PATHAK, MEDHA M · 2018 to 2022
$2.2M
Piezo1 Mobility Dynamics in MechanotransductionF31NS127594 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI LY, ALAN · 2022 to 2023
$84k
NCCIH NIH HHS DP2 AT010376NINDS NIH HHS F31 NS127594NINDS NIH HHS R01 NS109810NINDS NIH HHS T32 NS082174
6 · The paper itself

Abstract

PIEZO1 is critical to numerous physiological processes, transducing diverse mechanical stimuli into electrical and chemical signals. Recent studies underscore the importance of visualizing endogenous PIEZO1 activity and localization to understand its functional roles. To enable physiologically and clinically relevant studies on human PIEZO1, we genetically engineered human induced pluripotent stem cells (hiPSCs) to express a HaloTag fused to endogenous PIEZO1. Combined with advanced imaging, our chemogenetic platform allows precise visualization of PIEZO1 localization dynamics in various cell types. Furthermore, the PIEZO1-HaloTag hiPSC technology facilitates the non-invasive monitoring of channel activity across diverse cell types using Ca

Identifiers

PMID38187535
PMCPMC10769387
OpenAlexW4390154992

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.