Evidence map›Paper›PMID 38184717›Full record

ArticleCommunications biology2024

High quality genomes produced from single MinION flow cells clarify polyploid and demographic histories of critically endangered Fraxinus (ash) species.

Steven J Fleck, Crystal Tomlin, Flavio Augusto da Silva Coelho, Michaela Richter, Erik S Danielson, Nathan Backenstose, Trevor Krabbenhoft, Charlotte Lindqvist, Victor A Albert

Open access · goldAbstract read
In one paragraph

Article in Communications biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.5field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Steven J FleckDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA. sjfleck@buffalo.edu.ORCID 0000-0001-5370-2258
Crystal TomlinDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA.
Flavio Augusto da Silva CoelhoDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA.ORCID 0000-0003-0236-7044
Michaela RichterDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA.ORCID 0000-0001-7537-4902
Erik S DanielsonWestern New York Land Conservancy, East Aurora, NY, 14052, USA.
Nathan BackenstoseDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA.ORCID 0000-0002-4793-704X
Trevor KrabbenhoftDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA.ORCID 0000-0002-7680-5169
Charlotte LindqvistDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA.ORCID 0000-0002-4190-727X
Victor A AlbertDepartment of Biological Sciences, University at Buffalo, Buffalo, NY, 14260, USA. vaalbert@buffalo.edu.ORCID 0000-0002-0262-826X
University at Buffalo, State University of New York · USCentral Park Conservancy · US

Funding

National Science Foundation (NSF) DEB-2030871National Science Foundation (NSF) DEB-2139311
6 · The paper itself

Abstract

With populations of threatened and endangered species declining worldwide, efforts are being made to generate high quality genomic records of these species before they are lost forever. Here, we demonstrate that data from single Oxford Nanopore Technologies (ONT) MinION flow cells can, even in the absence of highly accurate short DNA-read polishing, produce high quality de novo plant genome assemblies adequate for downstream analyses, such as synteny and ploidy evaluations, paleodemographic analyses, and phylogenomics. This study focuses on three North American ash tree species in the genus Fraxinus (Oleaceae) that were recently added to the International Union for Conservation of Nature (IUCN) Red List as critically endangered. Our results support a hexaploidy event at the base of the Oleaceae as well as a subsequent whole genome duplication shared by Syringa, Osmanthus, Olea, and Fraxinus. Finally, we demonstrate the use of ONT long-read sequencing data to reveal patterns in demographic history.

Indexed as

FraxinusAnimalsDemographyGenome, PlantGenomicsPolyploidy

Identifiers

PMID38184717
PMCPMC10771460
OpenAlexW4390639573

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.