Evidence map›Paper›PMID 38184486›Full record

ArticleBiotechnology letters2024

Highly efficient biosynthesis of salidroside by a UDP-glucosyltransferase-catalyzed cascade reaction.

Guosi Li, Qilin Xu, Nan Hu, Xinyang Liu, Yiqi Jiang, Hailong Xue, Yongjun Zang, Fucheng Zhu

Abstract read
PubMed Publisher
In one paragraph

Article in Biotechnology letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Efficient production of hydroxysalidroside inSynthetic and systems biotechnology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Guosi LiAnhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China. liguosi1989@163.com.ORCID http://orcid.org/0000-0003-4677-2666
Qilin XuAnhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China.
Nan HuAnhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China.
Xinyang LiuAnhui Engineering Research Center for Eco-Agriculture of Traditional Chinese Medicine, Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, 237012, Anhui, China.
Yiqi JiangKey Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, Zhejiang, China.
Hailong XueKey Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, Zhejiang, China.
Yongjun ZangAnhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China.
Fucheng ZhuAnhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China. fucheng323@163.com.

Funding

Fund of Generic Technology Research center for Anhui Traditional Chinese Medicine Industry AHTCMGTRC-2023-09National Natural Science Foundation of China 32201979Natural Science Foundation of Higher Education Institutions of Anhui Province 2023AH052649Open Fund of Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources TCMRPSU-2022-03University Research Project of Anhui Province 2022AH051682
6 · The paper itself

Abstract

objectiveSalidroside is an important plant-derived aromatic compound with diverse biological properties. The main objective of this study was to synthesize salidroside from tyrosol using UDP-glucosyltransferase (UGT) with in situ regeneration of UDP-glucose (UDPG).

resultsThe UDP-glucosyltransferase 85A1 (UGT85A1) from Arabidopsis thaliana, which showed high activity and regioselectivity towards tyrosol, was selected for the production of salidroside. Then, an in vitro cascade reaction for in situ regeneration of UDPG was constructed by coupling UGT85A1 to sucrose synthase from Glycine max (GmSuSy). The optimal UGT85A1-GmSuSy activity ratio of 1:2 was determined to balance the efficiency of salidroside production and UDP-glucose regeneration. Different cascade reaction conditions for salidroside production were also determined. Under the optimized condition, salidroside was produced at a titer of 6.0 g/L with a corresponding molar conversion of 99.6% and a specific productivity of 199.1 mg/L/h in a continuous feeding reactor.

conclusionThis is the highest salidroside titer ever reported so far using biocatalytic approach.

Indexed as

GlucosidesGlucosyltransferasesPhenolsUridine Diphosphate GlucoseBiocatalysisGlucosePhenylethyl Alcohol4-hydroxyphenylethanolGlucoseGlucosidesGlucosyltransferasesPhenolsPhenylethyl AlcoholrhodiolosideUridine Diphosphate GlucoseSalidrosideSucrose synthaseTyrosolUDP-glucose regenerationUDP-glucosyltransferase

Identifiers

PMID38184486

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.