Evidence map›Paper›PMID 38183989›Full record

ArticleCell chemical biology2024

An acyl-CoA thioesterase is essential for the biosynthesis of a key dauer pheromone in C. elegans.

Subhradeep Bhar, Chi-Su Yoon, Kevin Mai, Jungsoo Han, Dilip V Prajapati, Yuting Wang, Candy L Steffen, Laura S Bailey, Kari B Basso, Rebecca A Butcher

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell chemical biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Subhradeep BharDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Chi-Su YoonDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Kevin MaiDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Jungsoo HanDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Dilip V PrajapatiDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Yuting WangDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Candy L SteffenDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Laura S BaileyDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Kari B BassoDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA.
Rebecca A ButcherDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA. Electronic address: butcher@chem.ufl.edu.
University of Florida · US

Funding

Small-molecule signals controlling nematode developmentR35GM144076 · NIGMS · UNIVERSITY OF FLORIDA · PI Rebecca A Butcher · 2022 to 2026
$2.1M
Acquisition of a Q Exactive HF Hybrid Quadrupole-OrbitrapS10OD021758 · OD · UNIVERSITY OF FLORIDA · PI BASSO, KARI B · 2017 to 2017
$600k
NIGMS NIH HHS R35 GM144076NIH HHS S10 OD021758
6 · The paper itself

Abstract

Methyl ketone (MK)-ascarosides represent essential components of several pheromones in Caenorhabditis elegans, including the dauer pheromone, which triggers the stress-resistant dauer larval stage, and the male-attracting sex pheromone. Here, we identify an acyl-CoA thioesterase, ACOT-15, that is required for the biosynthesis of MK-ascarosides. We propose a model in which ACOT-15 hydrolyzes the β-keto acyl-CoA side chain of an ascaroside intermediate during β-oxidation, leading to decarboxylation and formation of the MK. Using comparative metabolomics, we identify additional ACOT-15-dependent metabolites, including an unusual piperidyl-modified ascaroside, reminiscent of the alkaloid pelletierine. The β-keto acid generated by ACOT-15 likely couples to 1-piperideine to produce the piperidyl ascaroside, which is much less dauer-inducing than the dauer pheromone, asc-C6-MK (ascr#2, 1). The bacterial food provided influences production of the piperidyl ascaroside by the worm. Our work shows how the biosynthesis of MK- and piperidyl ascarosides intersect and how bacterial food may impact chemical signaling in the worm.

Indexed as

Caenorhabditis elegansPheromonesAnimalsCaenorhabditis elegans ProteinsThiolester HydrolasesCaenorhabditis elegans ProteinsPheromonesThiolester HydrolasesACOT-15acyl-CoA thioesteraseanaferineascarosideascarosidesascr#2attractionavoidancebeta-oxidationCaenorhabditis elegansdauerhydroxylaminelifespanmetabolomicsmethyl ketonenatural productspelletierinepheromonepiperideinepiperidinestructure elucidation

Identifiers

PMID38183989
PMCPMC11102344
OpenAlexW4390619426

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.