ArticlePlant methods2024
Mitochondrial DNA editing in potato through mitoTALEN and mitoTALECD: molecular characterization and stability of editing events.
Article in Plant methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- AI-Assisted Spatial Metabolic Engineering in Plants: Integrating Flux Design, Spatial Omics, and Synthetic Biology.Metabolites · 2026Review
- Plant responses to cold stress: molecular and physiological adaptive mechanisms with breeding advances.Frontiers in plant science · 2026Review
- Advancements in genomic crop techniques and considerations for regulation and food safety.Transgenic research · 2025Review
- Genome modification in plant mitochondria.Plant physiology · 2025Review
- Mitochondrial gene editing and allotopic expression unveil the role of orf125 in the induction of male fertility in some Solanum spp. hybrids and in the evolution of the common potato.Plant biotechnology journal · 2025Article
- Engineering cold resilience: implementing gene editing tools for plant cold stress tolerance.Planta · 2024Review
- Knocking out of the Mitochondrial Gene orf218 Using mitoTALECD Restores Cytoplasmic Male Sterility in Eggplant.Physiologia plantarumArticle
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
backgroundThe aim of this study was to evaluate and characterize the mutations induced by two TALE-based approaches, double-strand break (DSB) induction by the FokI nuclease (mitoTALEN) and targeted base editing by the DddA cytidine deaminase (mitoTALECD), to edit, for the first time, the mitochondrial genome of potato, a vegetatively propagated crop. The two methods were used to knock out the same mitochondrial target sequence (orf125).
resultsTargeted chondriome deletions of different sizes (236-1066 bp) were induced by mitoTALEN due to DSB repair through ectopic homologous recombination of short direct repeats (11-12 bp) present in the target region. Furthermore, in one case, the induced DSB and subsequent repair resulted in the amplification of an already present substoichiometric molecule showing a 4288 bp deletion spanning the target sequence. With the mitoTALECD approach, both nonsense and missense mutations could be induced by base substitution. The deletions and single nucleotide mutations were either homoplasmic or heteroplasmic. The former were stably inherited in vegetative offspring.
conclusionsBoth editing approaches allowed us to obtain plants with precisely modified mitochondrial genomes at high frequency. The use of the same plant genotype and mtDNA region allowed us to compare the two methods for efficiency, accuracy, type of modifications induced and stability after vegetative propagation.
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