Evidence map›Paper›PMID 38182732›Full record

ArticleCommunications biology2024

SNAP-25, but not SNAP-23, is essential for photoreceptor development, survival, and function in mice.

Mengjia Huang, Chun Hin Chow, Akshay Gurdita, Hidekiyo Harada, Victor Q B Pham Truong, Sarah Eide, Hong-Shuo Sun, Zhong-Ping Feng, Philippe P Monnier, Valerie A Wallace and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.0field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

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  6. Syntaxin 3B: A SNARE Protein Required for Vision.International journal of molecular sciences · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Mengjia HuangDivision of Experimental & Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Chun Hin ChowDivision of Experimental & Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Akshay GurditaDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Hidekiyo HaradaDonald K. Johnson Eye Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.
Victor Q B Pham TruongDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Sarah EideDepartment of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Hong-Shuo SunDepartment of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Zhong-Ping FengDepartment of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Philippe P MonnierDepartment of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Valerie A WallaceDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Shuzo SugitaDivision of Experimental & Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada. Shuzo.Sugita@uhnresearch.ca.ORCID 0000-0002-9182-873X
University Health Network · CAUniversity of Toronto · CA

Funding

Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada) RGPIN 2020 07139
6 · The paper itself

Abstract

SNARE-mediated vesicular transport is thought to play roles in photoreceptor glutamate exocytosis and photopigment delivery. However, the functions of Synaptosomal-associated protein (SNAP) isoforms in photoreceptors are unknown. Here, we revisit the expression of SNAP-23 and SNAP-25 and generate photoreceptor-specific knockout mice to investigate their roles. Although we find that SNAP-23 shows weak mRNA expression in photoreceptors, SNAP-23 removal does not affect retinal morphology or vision. SNAP-25 mRNA is developmentally regulated and undergoes mRNA trafficking to photoreceptor inner segments at postnatal day 9 (P9). SNAP-25 knockout photoreceptors develop normally until P9 but degenerate by P14 resulting in severe retinal thinning. Photoreceptor loss in SNAP-25 knockout mice is associated with abolished electroretinograms and vision loss. We find mistrafficked photopigments, enlarged synaptic vesicles, and abnormal synaptic ribbons which potentially underlie photoreceptor degeneration. Our results conclude that SNAP-25, but not SNAP-23, mediates photopigment delivery and synaptic functioning required for photoreceptor development, survival, and function.

Indexed as

Photoreceptor Cells, VertebrateQb-SNARE ProteinsQc-SNARE ProteinsSynaptosomal-Associated Protein 25AnimalsBiological TransportCytoskeletonGlutamic AcidMiceMice, KnockoutRNA, MessengerGlutamic AcidQb-SNARE ProteinsQc-SNARE ProteinsRNA, MessengerSnap23 protein, mouseSnap25 protein, mouseSynaptosomal-Associated Protein 25

Identifiers

PMID38182732
PMCPMC10770054
OpenAlexW4390617173

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.