Evidence map›Paper›PMID 38174044›Full record

ArticleFrontiers in genetics2023

Comparative miRNA expression profile analysis of porcine ovarian follicles: new insights into the initiation mechanism of follicular atresia.

Jingge Liu, Caibo Ning, Jinbi Zhang, Shiyong Xu, Jiege Wu, Chenyu Tao, Fanhua Ma, Qing Chen, Zengxiang Pan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in genetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Jingge Liu *College of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, China.
Caibo Ning *College of Animal Science and Technology, Nanjing Agriculture University, Nanjing, China.
Jinbi ZhangCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, China.
Shiyong XuCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, China.
Jiege WuCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, China.
Chenyu TaoCollege of Animal Science and Technology, Hebei Agricultural University, Baoding, China.
Fanhua MaCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, China.
Qing ChenCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, China.
Zengxiang PanCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing, China.
Jinling Institute of Technology · CNNanjing Agricultural University · CNHebei Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Follicular atresia occurs in every stage of ovarian development, which is relevant to female fertility. In the past decade, increasing studies have confirmed that miRNAs, a class of short non-coding RNAs, play an important role in follicular atresia by post-transcription regulation of their target genes. However, the function of miRNAs on follicular atresia initiation is unknown. In the present study, high-throughput small RNA sequencing was performed to analyze differential miRNA expression profiles between healthy (HF) follicles and early atretic (EAF) follicles. A total of 237 conserved miRNA were detected, and the miR-143 is the highest expressed in follicles. Meanwhile, we also found wide sequence variations (isomiRs) in porcine ovarian miRNA, including in 5'un-translation region, core seed sequences and 3'untranslation region. Furthermore, we identified 22 differentially expressed miRNAs in EAF groups compared to HF group, of which 3 miRNAs were upregulated, as well as 19 miRNAs were downregulated, and then the RT-PCR was performed to validate these profiles. The target genes of these differentially expressed miRNAs were predicted by using miRwalk, miRDB, and Targetscan database, respectively. Moreover, the gene ontology and KEGG pathway enrichment established that the regulating functions and signaling pathways of these miRNAs contribute to follicular atresia initiation and cell fate. In conclusion, this study provides new insights into the changes of miRNAs in early atretic follicles to demonstrate their molecular regulation in ovarian follicular atretic initiation.

Indexed as

follicle atresia initiationfunction analysismiRNAspig ovarysequencing

Identifiers

PMID38174044
PMCPMC10761487
OpenAlexW4390009076

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.