ArticleNature microbiology2024
The Vibrio cholerae CBASS phage defence system modulates resistance and killing by antifolate antibiotics.
Article in Nature microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- How bacterial immune systems sense phage infection.Nature reviews. Microbiology · 2026Review
- Members of theMicrobial genomics · 2026Article
- CBASS limits bacteriophage production while maintaining cell viability in Pseudomonas aeruginosa.Cell host & microbe · 2026Article
- Review
- DNA-intercalating antiphage molecules trigger abortive infection through mutual destruction and synergize with bacterial immunity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Bacterial defense systems: Mechanisms, homology to eukaryotic immune systems, and applications.Zoological research · 2026Review
- Article
- Deazaguanylation is a nucleobase-protein conjugation required for type IV CBASS immunity.Science (New York, N.Y.) · 2025Article
- Defence systems encoded by core genomic islands of seventh pandemicPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Heterologous expression of the M. abscessus CBASS system confers phage TM4 resistance in M. smegmatis.BMC microbiology · 2025Article
- Systematic screen uncovers regulator contributions to chemical cues in Escherichia coli.PLoS biology · 2025Article
- Cholera rapid diagnostic tests at the host-microbe interface: Key Considerations for Global Deployments.medRxiv : the preprint server for health sciences · 2025Article
- A large-scale type I CBASS antiphage screen identifies the phage prohead protease as a key determinant of immune activation and evasion.Cell host & microbe · 2024Article
- Reversible conjugation of a CBASS nucleotide cyclase regulates bacterial immune response to phage infection.Nature microbiology · 2024Article
- Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system.mSphere · 2023Article
- Activation of amBio · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Toxic bacterial modules such as toxin-antitoxin systems hold antimicrobial potential, though successful applications are rare. Here we show that in Vibrio cholerae the cyclic-oligonucleotide-based anti-phage signalling system (CBASS), another example of a toxic module, increases sensitivity to antifolate antibiotics up to 10×, interferes with their synergy and ultimately enables bacterial lysis by these otherwise classic bacteriostatic antibiotics. Cyclic-oligonucleotide production by the CBASS nucleotidyltransferase DncV upon antifolate treatment confirms full CBASS activation under these conditions, and suggests that antifolates release DncV allosteric inhibition by folates. Consequently, the CBASS-antifolate interaction is specific to CBASS systems with closely related nucleotidyltransferases and similar folate-binding pockets. Last, antifolate resistance genes abolish the CBASS-antifolate interaction by bypassing the effects of on-target antifolate activity, thereby creating potential for their coevolution with CBASS. Altogether, our findings illustrate how toxic modules can impact antibiotic activity and ultimately confer bactericidal activity to classical bacteriostatic antibiotics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.