ArticleNature plants2024
Microtubule-associated phase separation of MIDD1 tunes cell wall spacing in xylem vessels in Arabidopsis thaliana.
Article in Nature plants, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- A grass-specific structural myosin VIII regulates protoxylem development and hydraulic conductance in sorghum.Plant physiology · 2026Article
- Biomolecular condensates in plant stress and development: recent advances and emerging concepts.Journal of experimental botany · 2026Review
- REAP1/AtSWAP70 integrates RAB5 and ROP signalling during sexual reproduction.Nature plants · 2026Article
- Relationship Between Filament-Polymerizing Muscle Myosin and Droplets Generated by Liquid-Liquid Phase Separation.Chembiochem : a European journal of chemical biology · 2026Article
- Prediction of plant phase-separating proteins using positive-unlabeled learning.Genome biology · 2026Article
- CESA7 and microtubules pattern complex secondary cell walls in explosive fruit of Cardamine hirsuta.The Plant cell · 2026Article
- The role of VND transcription factors in xylem vessel development and secondary wall formation.The New phytologist · 2025Review
- An ABA-ROP toggle switch orchestrates xylem differentiation and cell wall patterning.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels.Nature communications · 2025Article
- Time-lapse confocal imaging helps to reveal a secret behind gynoecium development.Quantitative plant biology · 2025Article
- Microtubule flexibility, microtubule-based nucleation and ROP pattern co-alignment enhance protoxylem microtubule patterning.Quantitative plant biology · 2025Article
- The Turing heritage for plant biology: all spots and stripes?Quantitative plant biology · 2025Article
- Plants reshape protoxylem through tubulin adjustment.Plant physiology · 2024Article
- RHO OF PLANTS signalling and the activating ROP GUANINE NUCLEOTIDE EXCHANGE FACTORS: specificity in cellular signal transduction in plants.Journal of experimental botany · 2024Review
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Properly patterned cell walls specify cellular functions in plants. Differentiating protoxylem and metaxylem vessel cells exhibit thick secondary cell walls in striped and pitted patterns, respectively. Cortical microtubules are arranged in distinct patterns to direct cell wall deposition. The scaffold protein MIDD1 promotes microtubule depletion by interacting with ROP GTPases and KINESIN-13A in metaxylem vessels. Here we show that the phase separation of MIDD1 fine-tunes cell wall spacing in protoxylem vessels in Arabidopsis thaliana. Compared with wild-type, midd1 mutants exhibited narrower gaps and smaller pits in the secondary cell walls of protoxylem and metaxylem vessel cells, respectively. Live imaging of ectopically induced protoxylem vessels revealed that MIDD1 forms condensations along the depolymerizing microtubules, which in turn caused massive catastrophe of microtubules. The MIDD1 condensates exhibited rapid turnover and were susceptible to 1,6-hexanediol. Loss of ROP abolished the condensation of MIDD1 and resulted in narrow cell wall gaps in protoxylem vessels. These results suggest that the microtubule-associated phase separation of MIDD1 facilitates microtubule arrangement to regulate the size of gaps in secondary cell walls. This study reveals a new biological role of phase separation in the fine-tuning of cell wall patterning.
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