Evidence map›Paper›PMID 38170326›Full record

ArticleMolecular biology reports2024

Unveiling the therapeutic potential of thymol from Nigella sativa L. seed: selective anticancer action against human breast cancer cells (MCF-7) through down-regulation of Cyclin D1 and proliferative cell nuclear antigen (PCNA) expressions.

V Vahitha, Growther Lali, Saradh Prasad, Ponmurugan Karuppiah, Gopalu Karunakaran, Mohamad S AlSalhi

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
7.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

V VahithaDepartment of Microbiology, Hindusthan College of Arts & Science, Coimbatore, Tamil Nadu, 641028, India.
Growther LaliDepartment of Microbiology, Hindusthan College of Arts & Science, Coimbatore, Tamil Nadu, 641028, India. lallgrowther@hicas.ac.in.
Saradh PrasadDepartment of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Ponmurugan KaruppiahDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Gopalu KarunakaranDepartment of Fine Chemistry, Institute for Applied Chemistry, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea.
Mohamad S AlSalhiDepartment of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia. malsalhi@ksu.edu.sa.
King Saud University · SAHindustan Institute of Technology and Science · INSeoul National University of Science and Technology · KR

Funding

King Saud University RSPD2023R723
6 · The paper itself

Abstract

backgroundBreast adenocarcinoma cells (MCF-7) are characterized by the overexpression of apoptotic marker genes and proliferative cell nuclear antigen (PCNA), which promote cancer cell proliferation. Thymol, derived from Nigella sativa (NS), has been investigated for its potential anti-proliferative and anticancer properties, especially its ability to suppress Cyclin D1 and PCNA expression, which are crucial in the proliferation of cancer cells.

methodsThe cytotoxicity of thymol on MCF-7 cells was assessed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release methods. Thymol was tested at increasing concentrations (0-1000 µM) to evaluate its impact on MCF-7 cell growth. Additionally, Cyclin D1 and PCNA gene expression in thymol-treated and vehicle control groups of MCF-7 were quantified using real-time Polymerase Chain Reaction (RT-qPCR). Protein-ligand interactions were also investigated using the CB-Dock2 server.

resultsThymol significantly inhibited MCF-7 cell growth, with a 50% inhibition observed at 200 µM. The gene expression of Cyclin D1 and PCNA was down-regulated in the thymol-treated group relative to the vehicle control. The experimental results were verified through protein-ligand interaction investigations.

conclusionsThymol, extracted from NS, demonstrated specific cytotoxic effects on MCF-7 cells by suppressing the expression of Cyclin D1 and PCNA, suggesting its potential as an effective drug for MCF-7. However, additional in vivo research is required to ascertain its efficacy and safety in medical applications.

Indexed as

Breast NeoplasmsNigella sativaAntigens, NuclearCell ProliferationCyclin D1Down-RegulationFemaleHumansLigandsMCF-7 CellsProliferating Cell Nuclear AntigenThymolAntigens, NuclearCyclin D1LigandsProliferating Cell Nuclear AntigenThymolCyclin D1Gene expressionMCF-7MTTNigella sativaThymol

Identifiers

PMID38170326
OpenAlexW4390545758

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.