Evidence map›Paper›PMID 38168989›Full record

ArticleNature biotechnology2024

High-quality metagenome assembly from long accurate reads with metaMDBG.

Gaëtan Benoit, Sébastien Raguideau, Robert James, Adam M Phillippy, Rayan Chikhi, Christopher Quince

Abstract read
In one paragraph

Article in Nature biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.

0numbers the graph read from it
0cells of the map it votes in
103citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

103 citing papers in PubMed.

  1. Review
  2. Complete genome sequence ofMicrobiology resource announcements · 2026
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  8. Complete genome sequence ofMicrobiology resource announcements · 2026
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  9. Programmed DNA elimination was present in the last common ancestor ofbioRxiv : the preprint server for biology · 2026
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  12. Review
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43 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Gaëtan BenoitOrganisms and Ecosystems, Earlham Institute, Norwich, UK.
Sébastien RaguideauOrganisms and Ecosystems, Earlham Institute, Norwich, UK.ORCID http://orcid.org/0000-0001-8727-2604
Robert JamesGut Microbes and Health, Quadram Institute, Norwich, UK.
Adam M PhillippyGenome Informatics Section, National Human Genome Research Institute, Bethesda, MD, USA.ORCID http://orcid.org/0000-0003-2983-8934
Rayan ChikhiSequence Bioinformatics, Department of Computational Biology, Institut Pasteur, Paris, France.
Christopher QuinceOrganisms and Ecosystems, Earlham Institute, Norwich, UK. christopher.quince@earlham.ac.uk.ORCID http://orcid.org/0000-0003-1884-8440

Funding

Single-molecule sequence assembly and analysisZIAHG200398 · NHGRI · NATIONAL HUMAN GENOME RESEARCH INSTITUTE · PI PHILLIPPY, ADAM · 2016 to 2025
$15.9M
EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) 956229RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BB/CSP1720/1RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BB/N023285/1RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BBS/E/T/000PR9817RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BS/E/T/000PR9818RCUK | Medical Research Council (MRC) MR/S037195/1RCUK | Natural Environment Research Council (NERC) NE/T013230/1
6 · The paper itself

Abstract

We introduce metaMDBG, a metagenomics assembler for PacBio HiFi reads. MetaMDBG combines a de Bruijn graph assembly in a minimizer space with an iterative assembly over sequences of minimizers to address variations in genome coverage depth and an abundance-based filtering strategy to simplify strain complexity. For complex communities, we obtained up to twice as many high-quality circularized prokaryotic metagenome-assembled genomes as existing methods and had better recovery of viruses and plasmids.

Indexed as

MetagenomeMetagenomicsAlgorithmsHigh-Throughput Nucleotide SequencingSequence Analysis, DNASoftware

Identifiers

PMID38168989
PMCPMC11392814

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.