ArticleNature cell biology2024
Higher-order protein assembly controls kinetochore formation.
Article in Nature cell biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 21 citations in OpenAlex.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Probing Centromere Plasticity and Chromatin Remodeling Using Single-Molecule Techniques.Cell biology international · 2026Review
- Article
- Architecture and function of holocentric CENP-A-independent inner kinetochores.Science advances · 2026Article
- The dynamics of centromere assembly and disassembly during quiescence.The Journal of cell biology · 2026Article
- Mammalian metaphase kinetochores are elastic and require condensin for robust structure and function.bioRxiv : the preprint server for biology · 2025Article
- Decoding necrosome assembly: harmonizing signal amplification and attenuation through optimal RIP3 stoichiometry.Nature communications · 2025Article
- Article
- 40 years of CENP-A: the foundation of a new era of centromere biology.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Designing protein-based artificial kinetochores as decoys to prevent meiotic errors in oocytes.Nature cell biology · 2025Article
- DNA methylation influences human centromere positioning and function.Nature genetics · 2025Article
- The dynamics of centromere assembly and disassembly during quiescence.bioRxiv : the preprint server for biology · 2025Article
- Cohesin-mediated stabilization of the CCAN complex at kinetochores in mitosis.Current biology : CB · 2025Article
- Binding site maturation modulated by molecular density underlies Ndc80 binding to kinetochore receptor CENP-T.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Bridging the gap: unravelling plant centromeres in the telomere-to-telomere era.The New phytologist · 2024Review
- A noncanonical GTPase signaling mechanism controls exit from mitosis in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Regulation of outer kinetochore assembly during meiosis I and II by CENP-A and KNL-2/M18BP1 in C. elegans oocytes.Current biology : CB · 2024Article
- A noncanonical GTPase signaling mechanism controls exit from mitosis in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- The structure, function, and evolution of plant centromeres.Genome research · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
To faithfully segregate chromosomes during vertebrate mitosis, kinetochore-microtubule interactions must be restricted to a single site on each chromosome. Prior work on pair-wise kinetochore protein interactions has been unable to identify the mechanisms that prevent outer kinetochore formation in regions with a low density of CENP-A nucleosomes. To investigate the impact of higher-order assembly on kinetochore formation, we generated oligomers of the inner kinetochore protein CENP-T using two distinct, genetically engineered systems in human cells. Although individual CENP-T molecules interact poorly with outer kinetochore proteins, oligomers that mimic centromeric CENP-T density trigger the robust formation of functional, cytoplasmic kinetochore-like particles. Both in cells and in vitro, each molecule of oligomerized CENP-T recruits substantially higher levels of outer kinetochore components than monomeric CENP-T molecules. Our work suggests that the density dependence of CENP-T restricts outer kinetochore recruitment to centromeres, where densely packed CENP-A recruits a high local concentration of inner kinetochore proteins.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.