Evidence map›Paper›PMID 38168485›Full record

ArticleScientific reports2024

A novel portable flip-phone based visual behaviour assay for zebrafish.

Vanessa Rodwell, Annabel Birchall, Ha-Jun Yoon, Helen J Kuht, William H J Norton, Mervyn G Thomas

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.0field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Vanessa RodwellThe University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSB, PO Box 65, Leicester, LE2 7LX, UK.
Annabel BirchallThe University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSB, PO Box 65, Leicester, LE2 7LX, UK.
Ha-Jun YoonThe University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSB, PO Box 65, Leicester, LE2 7LX, UK.
Helen J KuhtThe University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSB, PO Box 65, Leicester, LE2 7LX, UK.
William H J NortonDepartment of Genetics and Genome Biology, University of Leicester, Leicester, LE1 7RH, UK.
Mervyn G ThomasThe University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSB, PO Box 65, Leicester, LE2 7LX, UK. mt350@le.ac.uk.
Ulverscroft (United Kingdom) · GBUniversity of Leicester · GB

Funding

Academy of Medical Sciences SGL021/1066Wellcome Trust
6 · The paper itself

Abstract

The optokinetic reflex (OKR) serves as a vital index for visual system development in early life, commonly observed within the first six months post-birth in humans. Zebrafish larvae offer a robust and convenient model for OKR studies due to their rapid development and manageable size. Existing OKR assays often involve cumbersome setups and offer limited portability. In this study, we present an innovative OKR assay that leverages the flexible screen of the Samsung Galaxy Z Flip to optimize setup and portability. We conducted paired slow-phase velocity measurements in 5-day post-fertilization (dpf) zebrafish larvae (n = 15), using both the novel flip-phone-based assay and a traditional liquid-crystal display (LCD) arena. Utilizing Bland-Altman plots, we assessed the agreement between the two methods. Both assays were efficacious in eliciting OKR, with eye movement analysis indicating high tracking precision in the flip-phone-based assay. No statistically significant difference was observed in slow-phase velocities between the two assays (p = 0.40). Our findings underscore the feasibility and non-inferiority of the flip-phone-based approach, offering streamlined assembly, enhanced portability, and the potential for cost-effective alternatives. This study contributes to the evolution of OKR assay methodologies, aligning them with emerging research paradigms.

Indexed as

Nystagmus, OptokineticZebrafishAnimalsHumansReflex

Identifiers

PMID38168485
PMCPMC10762252
OpenAlexW4390512244

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.