Evidence map›Paper›PMID 38168382›Full record

ArticleResearch square2023

Unravelling the complexities of DNA-PK activation by structure-based mutagenesis.

Christopher J Buehl, Noah J Goff, Mariia Mikhova, Steven W Hardwick, Thomas L Blundell, Mauro Modesti, Jens C Schmidt, Amanda Chaplin, Katheryn Meek

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Christopher J BuehlCollege of Veterinary Medicine, Department of Microbiology & Molecular Genetics, Department of Pathobiology & Diagnostic Investigation, Michigan State University, East Lansing, MI 48824, USA.
Noah J GoffCollege of Veterinary Medicine, Department of Microbiology & Molecular Genetics, Department of Pathobiology & Diagnostic Investigation, Michigan State University, East Lansing, MI 48824, USA.
Mariia MikhovaDepartment of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, U.S.A.
Steven W HardwickCryoEM Facility, Department of Biochemistry, University of Cambridge, Sanger Building, Tennis Court Road, Cambridge CB2 1GA, United Kingdom.ORCID https://orcid.org/0000-0001-9246-1864
Thomas L BlundellDepartment of Biochemistry, University of Cambridge, Sanger Building, Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.ORCID https://orcid.org/0000-0002-2708-8992
Mauro ModestiCancer Research Center of Marseille, Department of Genome Integrity, CNRS UMR7258, Inserm U1068, Institut Paoli-Calmettes, Aix Marseille Univ, Marseille, France.ORCID https://orcid.org/0000-0002-4964-331X
Jens C SchmidtInstitute for Quantitative Health Sciences and Engineering, Michigan State University, East Lansing, MI, U.S.A.
Amanda ChaplinCryoEM Facility, Department of Biochemistry, University of Cambridge, Sanger Building, Tennis Court Road, Cambridge CB2 1GA, United Kingdom.ORCID https://orcid.org/0000-0002-9606-8346
Katheryn MeekCollege of Veterinary Medicine, Department of Microbiology & Molecular Genetics, Department of Pathobiology & Diagnostic Investigation, Michigan State University, East Lansing, MI 48824, USA.ORCID https://orcid.org/0000-0002-0454-4414
Michigan State University · USUniversity of Cambridge · GBInstitut Pprime · FRUniversity of Leicester · GB

Funding

Relevant Targets of the DNA Dependent Protein KinaseR01AI048758 · NIAID · MICHIGAN STATE UNIVERSITY · PI MEEK, KATHERYN D · 2000 to 2016
$5.2M
Defining the mechanistic basis of ATM’s impact on VDJ recombinationR01AI147634 · NIAID · MICHIGAN STATE UNIVERSITY · PI MEEK, KATHERYN D, YU, KEFEI · 2019 to 2024
$1.9M
NIAID NIH HHS R01 AI048758NIAID NIH HHS R01 AI147634
6 · The paper itself

Abstract

It has been known for decades that the DNA-dependent protein kinase (DNA-PK) is only an active serine/threonine protein kinase when it is bound to a DNA double-stranded end; still, the molecular details of how this activation is achieved have remained elusive. The recent surge in structural information for DNA-PK complexes has provided valuable insights into the process of DNA end recognition by DNA-PK. A particularly intriguing feature of this kinase is a region of the protein that can transition from a seemingly structurally disordered state to a single alpha-helix that traverses down the DNA binding cradle. The DNA-PK bound DNA end of the DNA substrate engages with and appears to split around this helix which has been named the

Identifiers

PMID38168382
PMCPMC10760257
OpenAlexW4389664977

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.