Evidence map›Paper›PMID 38168332›Full record

ArticlebioRxiv : the preprint server for biology2023

Glioma Cells Secrete Collagen VI to Facilitate Invasion.

Junghwa Cha, Erika A Ding, Emily M Carvalho, Annabelle Fowler, Manish K Aghi, Sanjay Kumar

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 6 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Junghwa ChaDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA.
Erika A DingDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Emily M CarvalhoDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Annabelle FowlerDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA.
Manish K AghiDepartment of Neurosurgery, University of California San Francisco, San Francisco, CA 94143, USA.ORCID 0000-0002-2949-2227
Sanjay KumarDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA.ORCID 0000-0002-9996-4883
University of California, Berkeley · USUniversity of California, San Francisco · US

Funding

Tumor Evolution and Metastasis ProgramP30CA016672 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI Fabien Delerue · 1985 to 2026
$290.8M
Tropism Enhanced Oncolytic Adenovirus for the Treatment of Brain TumorsP50CA127001 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI Juan Fueyo · 2008 to 2026
$41.2M
Biophysical Control of Cell Form and Function by Single Actomyosin Stress Fibers: Instrument SupplementR01GM122375 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI KUMAR, SANJAY · 2017 to 2025
$2.7M
A Novel Adenoviral-Permissive, Immunocompetent Hamster Model to Evaluate Oncolytic Adenoviral Therapy for GlioblastomaR01CA247970 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI LANG, FREDERICK F · 2020 to 2024
$2.2M
Modeling and druggable-genome screening of glioblastoma invasion using regional biopsy-guided biomaterials systemsR01CA227136 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI AGHI, MANISH · 2018 to 2022
$2.2M
Retroviral RLI immunomodulatory gene therapy for glioblastomaR01NS123808 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Manish Aghi · 2022 to 2026
$2.1M
MSC-Derived Exosomes and MicroRNA in Glioma TherapyR01CA214749 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI LANG, FREDERICK F · 2018 to 2022
$1.8M
Mechanisms of adhesion and invasion in hyaluronic acid matricesR01CA260443 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI KUMAR, SANJAY · 2021 to 2025
$1.8M
LTQ XL Linear Quadrupole Ion Trap Mass SpectrometerS10RR025622 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI KOHLSTAEDT, LORI · 2009 to 2009
$463k
NCI NIH HHS P30 CA016672NCI NIH HHS P50 CA127001NCI NIH HHS R01 CA214749NCI NIH HHS R01 CA227136NCI NIH HHS R01 CA247970NCI NIH HHS R01 CA260443NCRR NIH HHS S10 RR025622NIGMS NIH HHS R01 GM122375NINDS NIH HHS R01 NS123808
6 · The paper itself

Abstract

While glioblastoma (GBM) progression is associated with extensive extracellular matrix (ECM) secretion, the causal contributions of ECM secretion to invasion remain unclear. Here we investigate these contributions by combining engineered materials, proteomics, analysis of patient data, and a model of bevacizumab-resistant GBM. We find that GBM cells cultured in engineered 3D hyaluronic acid hydrogels secrete ECM prior to invasion, particularly in the absence of exogenous ECM ligands. Proteomic measurements reveal extensive secretion of collagen VI, and collagen VI-associated transcripts are correspondingly enriched in microvascular proliferation regions of human GBMs. We further show that bevacizumab-resistant GBM cells deposit more collagen VI than their responsive counterparts, which is associated with marked cell-ECM stiffening. COL6A3 deletion in GBM cells reduces invasion, β-catenin signaling, and expression of mesenchymal markers, and these effects are amplified in hypoxia. Our studies strongly implicate GBM cell-derived collagen VI in microenvironmental remodeling to facilitate invasion.

Indexed as

Collagen VIECM remodelingECM stiffeningGlioblastomaHyaluronic acid

Identifiers

PMID38168332
PMCPMC10760023
OpenAlexW4389609506

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.