Evidence map›Paper›PMID 38167969›Full record

ArticleInternational microbiology : the official journal of the Spanish Society for Microbiology2024

Euglena mutabilis exists in a FAB consortium with microbes that enhance cadmium tolerance.

Emma Kaszecki, Daniel Palberg, Mikaella Grant, Sarah Griffin, Chetan Dhanjal, Michael Capperauld, R J Neil Emery, Barry J Saville

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In one paragraph

Article in International microbiology : the official journal of the Spanish Society for Microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Emma KaszeckiEnvironmental and Life Science Graduate Program, Trent University, Peterborough, ON, Canada.
Daniel PalbergEnvironmental and Life Science Graduate Program, Trent University, Peterborough, ON, Canada.
Mikaella GrantEnvironmental and Life Science Graduate Program, Trent University, Peterborough, ON, Canada.
Sarah GriffinForensic Science Department, Trent University, Peterborough, ON, Canada.
Chetan DhanjalDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Michael CapperauldDepartment of Biology, Trent University, Peterborough, ON, Canada.
R J Neil EmeryEnvironmental and Life Science Graduate Program, Trent University, Peterborough, ON, Canada.
Barry J SavilleEnvironmental and Life Science Graduate Program, Trent University, Peterborough, ON, Canada. barrysaville@trentu.ca.

Funding

Natural Sciences and Engineering Research Council of Canada RGPIN/6029-2019
6 · The paper itself

Abstract

backgroundSynthetic algal-fungal and algal-bacterial cultures have been investigated as a means to enhance the technological applications of the algae. This inclusion of other microbes has enhanced growth and improved stress tolerance of the algal culture. The goal of the current study was to investigate natural microbial consortia to gain an understanding of the occurrence and benefits of these associations in nature. The photosynthetic protist Euglena mutabilis is often found in association with other microbes in acidic environments with high heavy metal (HM) concentrations. This may suggest that microbial interactions are essential for the protist's ability to tolerate these extreme environments. Our study assessed the Cd tolerance of a natural fungal-algal-bacterial (FAB) association whereby the algae is E. mutabilis.

resultsThis study provides the first assessment of antibiotic and antimycotic agents on an E. mutabilis culture. The results indicate that antibiotic and antimycotic applications significantly decreased the viability of E. mutabilis cells when they were also exposed to Cd. Similar antibiotic treatments of E. gracilis cultures had variable or non-significant impacts on Cd tolerance. E. gracilis also recovered better after pre-treatment with antibiotics and Cd than did E. mutabilis. The recoveries were assessed by heterotrophic growth without antibiotics or Cd. In contrast, both Euglena species displayed increased chlorophyll production upon Cd exposure. PacBio full-length amplicon sequencing and targeted Sanger sequencing identified the microbial species present in the E. mutabilis culture to be the fungus Talaromyces sp. and the bacterium Acidiphilium acidophilum.

conclusionThis study uncovers a possible fungal, algal, and bacterial relationship, what we refer to as a FAB consortium. The members of this consortium interact to enhance the response to Cd exposure. This results in a E. mutabilis culture that has a higher tolerance to Cd than the axenic E. gracilis. The description of this interaction provides a basis for explore the benefits of natural interactions. This will provide knowledge and direction for use when creating or maintaining FAB interactions for biotechnological purposes, including bioremediation.

Indexed as

CadmiumEuglenaAnti-Bacterial AgentsAntifungal AgentsBacteriaFungiMicrobial ConsortiaAnti-Bacterial AgentsAntifungal AgentsCadmiumAlgal symbiosisBioremediationBiotechnologyCo-cultureFungal–algal–bacterial (FAB)Heavy metals

Identifiers

PMID38167969
PMCPMC11300505

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.