Evidence map›Paper›PMID 38167821›Full record

ArticleNature communications2024

Derivation of human primordial germ cell-like cells in an embryonic-like culture.

Sajedeh Nasr Esfahani, Yi Zheng, Auriana Arabpour, Agnes M Resto Irizarry, Norio Kobayashi, Xufeng Xue, Yue Shao, Cheng Zhao, Nicole L Agranonik, Megan Sparrow and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
8.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 35 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
  6. Mechanisms of human germ cell development.Nature reviews. Molecular cell biology · 2026
    Review
  7. Review
  8. Ascorbic acid enhancesFrontiers in cell and developmental biology · 2026
    Article
  9. In vitro models of the male reproductive system: applications for developmental and reproductive toxicology.Toxicological sciences : an official journal of the Society of Toxicology · 2025
    Review
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Review
  16. Stem cell-based models of early human development.Development (Cambridge, England) · 2025
    Review
  17. Pluripotent cell states and fates in human embryo models.Development (Cambridge, England) · 2025
    Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 5 institutions in 4 countries.

Sajedeh Nasr Esfahani *Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
Yi Zheng *Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0002-2685-3680
Auriana Arabpour *Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Agnes M Resto IrizarryDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
Norio KobayashiDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
Xufeng XueDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID 0000-0002-9379-8589
Yue ShaoInstitute of Biomechanics and Medical Engineering, Department of Engineering Mechanics, School of Aerospace Engineering, Tsinghua University, 100084, Beijing, China.ORCID 0000-0001-7548-3551
Cheng ZhaoDepartment of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynecology, Karolinska Instituet, 14186, Stockholm, Sweden.
Nicole L AgranonikDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Megan SparrowDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Timothy J HuntDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Jared FaithDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.ORCID 0000-0002-7823-3432
Mary Jasmine LaraDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Qiu Ya WuDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Sherman SilberInfertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, 63017, USA.
Sophie PetropoulosDepartment of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynecology, Karolinska Instituet, 14186, Stockholm, Sweden.ORCID 0000-0003-2293-8238
Ran YangDepartment of Cell and Molecular Biology, Karolinska Institutet, 171 77, Stockholm, Sweden.
Kenneth R ChienDepartment of Cell and Molecular Biology, Karolinska Institutet, 171 77, Stockholm, Sweden.ORCID 0000-0002-2759-8378
Amander T ClarkMolecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, 90095, USA. clarka@ucla.edu.ORCID 0000-0003-2483-3982
Jianping FuDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA. jpfu@umich.edu.ORCID 0000-0001-9629-6739
University of California, Los Angeles · USUniversity of Michigan · USKarolinska Institutet · SESt. Luke's Hospital · USTsinghua University · CN

Funding

TRAINING PROGRAM IN ORGANOGENESIST32HD007505 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PASCA DI MAGLIANO, MARINA, SPENCE, JASON · 1997 to 2021
$6.9M
Differentiating embryonic stem cells into developing germ lineR01HD079546 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CLARK, AMANDER · 2015 to 2024
$3.7M
Studying Origin of Human Primordial Germ Cells Using a Synthetic Stem Cell ModelR21HD100931 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FU, JIANPING · 2020 to 2021
$422k
Synthetic microfluidic synthesis of spinal cord tissues from human pluripotent stem cellsR21NS113518 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FU, JIANPING · 2019 to 2019
$422k
Amnion membrane organ-on-chip for modeling intra-amniotic infectionR21HD105192 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FU, JIANPING, ZHENG, YI · 2022 to 2023
$397k
Controlled generation of human embryoids using optogeneticsR21HD109635 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FU, JIANPING · 2022 to 2023
$336k
NICHD NIH HHS R01 HD079546NICHD NIH HHS R21 HD100931NICHD NIH HHS R21 HD105192NICHD NIH HHS R21 HD109635NICHD NIH HHS T32 HD007505NINDS NIH HHS R21 NS113518
6 · The paper itself

Abstract

Primordial germ cells (PGCs) are the embryonic precursors of sperm and eggs. They transmit genetic and epigenetic information across generations. Given the prominent role of germline defects in diseases such as infertility, detailed understanding of human PGC (hPGC) development has important implications in reproductive medicine and studying human evolution. Yet, hPGC specification remains an elusive process. Here, we report the induction of hPGC-like cells (hPGCLCs) in a bioengineered human pluripotent stem cell (hPSC) culture that mimics peri-implantation human development. In this culture, amniotic ectoderm-like cells (AMLCs), derived from hPSCs, induce hPGCLC specification from hPSCs through paracrine signaling downstream of ISL1. Our data further show functional roles of NODAL, WNT, and BMP signaling in hPGCLC induction. hPGCLCs are successfully derived from eight non-obstructive azoospermia (NOA) participant-derived hPSC lines using this biomimetic platform, demonstrating its promise for screening applications.

Indexed as

Pluripotent Stem CellsSemenCell DifferentiationCell LineGerm CellsHumansMaleSignal Transduction

Identifiers

PMID38167821
PMCPMC10762101
OpenAlexW4390498722

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.