ArticleMolecular horticulture2024
Agrobacterium rhizogenes-mediated marker-free transformation and gene editing system revealed that AeCBL3 mediates the formation of calcium oxalate crystal in kiwifruit.
Article in Molecular horticulture, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 23 citations in OpenAlex.
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- A RUBY-based in vivo hairy root induction system for rapid assessment of transformant and genome editing efficiency in potato.Journal, genetic engineering & biotechnology · 2026Article
- Kiwifruit genomics and applications: recent advances, current challenges, and future prospects.Horticulture research · 2026Article
- Allelic structural variation at the NLR25-1 locus enhances defense against Pseudomonas syringae in kiwifruit.Genome biology · 2026Article
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- Cryptochrome-mediated blue light regulates cell lignification via PbbHLH195 activation of the PbNSC in pear fruits.Molecular horticulture · 2025Article
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- Engineering Agrobacterium for improved plant transformation.The Plant journal : for cell and molecular biology · 2025Review
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19 authors at 2 institutions in 2 countries.
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Abstract
The transformation and gene editing of the woody species kiwifruit are difficult and time-consuming. The fast and marker-free genetic modification system for kiwifruit has not been developed yet. Here, we establish a rapid and efficient marker-free transformation and gene editing system mediated by Agrobacterium rhizogenes for kiwifruit. Moreover, a removing-root-tip method was developed to significantly increase the regeneration efficiency of transgenic hairy roots. Through A. rhizogenes-mediated CRISPR/Cas9 gene editing, the editing efficiencies of CEN4 and AeCBL3 achieved 55 and 50%, respectively. And several homozygous knockout lines for both genes were obtained. Our method has been successfully applied in the transformation of two different species of kiwifruit (Actinidia chinensis 'Hongyang' and A.eriantha 'White'). Next, we used the method to study the formation of calcium oxalate (CaOx) crystals in kiwifruit. To date, little is known about how CaOx crystal is formed in plants. Our results indicated that AeCBL3 overexpression enhanced CaOx crystal formation, but its knockout via CRISPR/Cas9 significantly impaired crystal formation in kiwifruit. Together, we developed a fast maker-free transformation and highly efficient CRISPR-Cas9 gene editing system for kiwifruit. Moreover, our work revealed a novel gene mediating CaOx crystal formation and provided a clue to elaborate the underlying mechanisms.
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