ArticleMethods in molecular biology (Clifton, N.J.)2024
Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-borne Pathogens in 48 or 96 Samples.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Research progress on emerging and important Tick-Borne pathogens.Frontiers in microbiology · 2026Review
- Detection of Multiple Microorganisms in Ruminant Ticks in Senegal Using High-Throughput Microfluidic Real-Time PCR.Transboundary and emerging diseases · 2026Review
- Wide spectrum of tick-borne pathogens in juvenile Ixodes ricinus collected from autumn-migrating birds in the Vistula River Valley, Poland.BMC veterinary research · 2024Article
- Update on tick-borne pathogens detection methods within ticks.Current research in parasitology & vector-borne diseases · 2024Article
Corrections and comments
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Authors and funding
2 authors.
Funding
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Abstract
Tick-borne pathogens (TBPs) are often detected through classical molecular tools (PCR, nested PCR, real-time PCR), but these are limited in terms of the number of targeted pathogens due to the volume of DNA available for analysis. To solve this problem, in 2014 we developed a new high-throughput method based on real-time microfluidic PCRs that can detect 48 or 96 pathogens in 48 or 96 samples in a single run, such as ten species from the Borrelia burgdorferi sensu lato group. We then used this technique for large-scale epidemiological studies of TBPs in tick and animal samples on an international scale through numerous collaborative projects.
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