Evidence map›Paper›PMID 38165462›Full record

ArticleMolecular biology reports2024

Molecular detection of Shiga toxin and extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates from sheep and goats.

Tsepo Ramatla, Mpho Tutubala, Tshepang Motlhaping, Lara de Wet, Prudent Mokgokong, Oriel Thekisoe, Kgaugelo Lekota

Open access · hybridAbstract read
In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 1 pooled it
14.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.

  1. Pooled it
  2. Shiga Toxin-ProducingAntibiotics (Basel, Switzerland) · 2026
    Review
  3. Article
  4. Article
  5. Article
  6. Antimicrobial-ResistantAntibiotics (Basel, Switzerland) · 2026
    Article
  7. Article
  8. Genotypic Profiling of Virulence and Antibiotic Resistance Genes ofInternational journal of microbiology · 2026
    Article
  9. Quantification of TotalMicroorganisms · 2025
    Article
  10. Prevalence and antimicrobial resistance patterns ofOne health (Amsterdam, Netherlands) · 2025
    Article
  11. Article
  12. Article
  13. Article
  14. Antimicrobial Resistance and Virulence Determinants ofAnimals : an open access journal from MDPI · 2025
    Article
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Tsepo RamatlaUnit for Environmental Sciences and Management, North West University, Potchefstroom, 2531, South Africa. ra21205450@gmail.com.
Mpho TutubalaUnit for Environmental Sciences and Management, North West University, Potchefstroom, 2531, South Africa.
Tshepang MotlhapingUnit for Environmental Sciences and Management, North West University, Potchefstroom, 2531, South Africa.
Lara de WetUnit for Environmental Sciences and Management, North West University, Potchefstroom, 2531, South Africa.
Prudent MokgokongUnit for Environmental Sciences and Management, North West University, Potchefstroom, 2531, South Africa.
Oriel ThekisoeUnit for Environmental Sciences and Management, North West University, Potchefstroom, 2531, South Africa.
Kgaugelo LekotaUnit for Environmental Sciences and Management, North West University, Potchefstroom, 2531, South Africa.
North-West University · ZA

Funding

NRF Thuthuka GUN: 129525
6 · The paper itself

Abstract

backgroundThe Shiga toxin (Stx)-producing Escherichia coli (STEC) have become important global public health concerns. This study investigated the prevalence, antimicrobial resistance profile, and extended-spectrum beta-lactamase-producing E. coli in sheep and goat faeces. METHODS AND

resultsA total of 53 E. coli isolates were confirmed by PCR targeting the uidA [β-D glucuronidase] gene. The Shiga toxin genes stx1 and stx2, as well as bfpA, vir, eaeA, lt and aafII virulence genes, were detected in this study. Of the 53 isolates confirmed to be STEC, 100% were positive for stx2 and 47.2% for stx1. Three isolates possessed a combination of stx1 + stx2 + eaeA, while four isolates harboured stx1 + stx2 + vir virulence genes. The isolates displayed phenotypic antimicrobial resistance against erythromycin (66.04%), colistin sulphate (43.4%), chloramphenicol (9.4%) and ciprofloxacin (1.9%). A total of 28.8% of the strains were phenotypically considered ESBL producers and contained the beta-lactamase bla

conclusionsOur findings highlight the importance of sheep and goats as sources of virulence genes and MDR E. coli. From a public health and veterinary medicine perspective, the characterization of ESBL producers originating from small livestock (sheep and goats) is crucial due to their close contact with humans.

Indexed as

Escherichia coliGoatsAnimalsAnti-Bacterial AgentsHumansHydrolasesSheepShiga ToxinsAnti-Bacterial AgentsHydrolasesShiga ToxinsAntimicrobial resistanceE. coliExtended-spectrum β-lactamasesShiga-toxinsVirulence factors

Identifiers

PMID38165462
PMCPMC10761393
OpenAlexW4390499400

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.