Evidence map›Paper›PMID 38164606›Full record

ArticleRNA (New York, N.Y.)2024

An easy tool to monitor the elemental steps of in vitro translation via gel electrophoresis of fluorescently labeled small peptides.

Valeriya I Marina, Medina Bidzhieva, Andrey G Tereshchenkov, Dmitry Orekhov, Vladislava E Sagitova, Nataliya V Sumbatyan, Vadim N Tashlitsky, Artem S Ferberg, Tinashe P Maviza, Pavel Kasatsky and 7 more

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In one paragraph

Article in RNA (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Characterization ofInternational journal of molecular sciences · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Valeriya I Marina *Center for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Skolkovo 121205, Russia.ORCID 0000-0002-3508-6858
Medina Bidzhieva *Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC "Kurchatov Institute," Gatchina 188300, Russia.
Andrey G TereshchenkovDepartment of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
Dmitry OrekhovR&D Department, VIC Animal Health, Severny, Belgorod Region 308519, Russia.
Vladislava E SagitovaDepartment of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
Nataliya V SumbatyanDepartment of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
Vadim N TashlitskyDepartment of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
Artem S FerbergDepartment of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia.
Tinashe P MavizaCenter for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Skolkovo 121205, Russia.
Pavel KasatskyMolecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC "Kurchatov Institute," Gatchina 188300, Russia.
Olga TolichevaMolecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC "Kurchatov Institute," Gatchina 188300, Russia.
Alena PaleskavaMolecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC "Kurchatov Institute," Gatchina 188300, Russia.
Vladimir I PolshakovFaculty of Fundamental Medicine, Lomonosov Moscow State University Moscow, Moscow 119991, Russia.
Ilya A OstermanCenter for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Skolkovo 121205, Russia.
Olga A DontsovaCenter for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Skolkovo 121205, Russia.
Andrey L KonevegaMolecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC "Kurchatov Institute," Gatchina 188300, Russia petya@genebee.msu.ru konevega_al@pnpi.nrcki.ru.ORCID 0000-0003-0125-7150
Petr V SergievCenter for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Skolkovo 121205, Russia petya@genebee.msu.ru konevega_al@pnpi.nrcki.ru.ORCID 0000-0001-8866-1863

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Several methods are available to visualize and assess the kinetics and efficiency of elemental steps of protein biosynthesis. However, each of these methods has its own limitations. Here, we present a novel, simple and convenient tool for monitoring stepwise in vitro translation initiated by BODIPY-Met-tRNA. Synthesis and release of very short, 1-7 amino acids, BODIPY-labeled peptides, can be monitored using urea-polyacrylamide gel electrophoresis. Very short BODIPY-labeled oligopeptides might be resolved this way, in contrast to widely used Tris-tricine gel electrophoresis, which is suitable to separate peptides larger than 1 kDa. The method described in this manuscript allows one to monitor the steps of translation initiation, peptide transfer, translocation, and termination as well as their inhibition at an unprecedented single amino acid resolution.

Indexed as

Boron CompoundsPeptidesRNA, Transfer, Amino AcylElectrophoresis, Polyacrylamide GelProtein BiosynthesisRNA, Transfer4,4-difluoro-4-bora-3a,4a-diaza-s-indaceneBoron CompoundsPeptidesRNA, TransferRNA, Transfer, Amino AcylBODIPYpeptidyl transferaseribosometerminationtranslationtRNA

Identifiers

PMID38164606
PMCPMC10870375

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.