ArticleThe world journal of men's health2024
Combined Analysis of the Transcriptome, Proteome and Metabolome in Human Cryopreserved Sperm.
Article in The world journal of men's health, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Biobanking biodiversity: recent strategies, challenges, and opportunities.The Journal of reproduction and development · 2026Review
- Trends in male semen parameters (2011-2018): a large-scale retrospective analysis of 5,886 cases based on the fifth edition WHO manual.Frontiers in public health · 2026Article
- Triclocarban impacts human sperm motility by inhibiting glycolysis and oxidative phosphorylation.Asian journal of andrology · 2025Article
- Sperm Membrane Stability: In-Depth Analysis from Structural Basis to Functional Regulation.Veterinary sciences · 2025Review
- Identification of hub genes associated with decreased fertility in male mice of advanced paternal age.Frontiers in cell and developmental biology · 2025Article
- Morphological Parameters of 29994 sperm in a fertile male population-based on Papanicolaou staining and SSA-II Plus.Frontiers in endocrinology · 2025Article
- The storage time of cryopreserved human spermatozoa does not affect pathways involved in fertility.Basic and clinical andrology · 2024Article
- Enhancement of Frozen-Thawed Human Sperm Quality with Zinc as a Cryoprotective Additive.Medical science monitor : international medical journal of experimental and clinical research · 2024Article
- A Pilot Analysis of Whole Transcriptome of Human Cryopreserved Sperm.International journal of molecular sciences · 2024Article
- Abnormalities in mitochondrial energy metabolism induced by cryopreservation negatively affect goat sperm motility.Frontiers in veterinary science · 2024Article
- Seminal plasma microbiomes, sperm parameters, and cryopreservation in a healthy fertile population.Frontiers in microbiology · 2024Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
purposeThis study aimed to identify the altered pathways and genes associated with freezing damage in human sperm during cryopreservation by multiomics analysis. MATERIALS AND
methodsFifteen fresh human semen samples were collected for transcriptomic analysis, and another 5 fresh human semen samples were obtained for metabolomic analysis. For each semen sample, 1 mL was cryopreserved, and another 1 mL was left untreated for paired design. The results were then combined with previously published proteomic results to identify key genes/pathways.
resultsCryopreservation significantly reduced sperm motility and mitochondrial structure. Transcriptomic analysis revealed altered mitochondrial function, including changes in tRNA-methyltransferase activity and adenosine tri-phosphate/adenosine di-phosphate transmembrane transporter activity. Metabolomic analysis showed that the citrate cycle in mitochondria was significantly altered. Combining transcriptomic, proteomic, and metabolomic analyses revealed 346 genes that were altered in at least two omics analyses. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that metabolic pathways were significantly altered and strongly associated with mitochondria. Five genes were altered in all three omics analyses:
conclusionsFive genes were identified by multiomics analysis in human cryopreserved sperm. These genes might have specific functions in cryopreservation. Explorations of the functions of these genes will be helpful for sperm cryopreservation and sperm motility improvement or even for reproduction in the future.
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