ArticleNature photonics2023
Bond-selective fluorescence imaging with single-molecule sensitivity.
Article in Nature photonics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- FILM: mapping organellar metabolism by mid-infrared photothermal-modulated fluorescence.Nature methods · 2026Article
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Resolving protein condensates and aggregates in vivo by boxcar-enhanced Fluorescence-detected mid-Infrared photothermaL Microscopy (FILM).Communications chemistry · 2026Article
- FILM: Mapping organellar metabolism by mid-infrared photothermal modulated fluorescence.ArXiv · 2026Article
- Article
- The Fantastic Single-Molecule Techniques.Chemical & biomedical imaging · 2026Review
- Far-field single-molecule vibrational spectroscopy and imaging.Chemical science · 2026Review
- Fluorescence-free single-molecule microscopy via electronic resonance stimulated Raman scattering.Nature communications · 2026Article
- Nonlinear optical microscopy with an obscuration-free, freeform reflective objective.Biomedical optics express · 2025Article
- Broadband Fourier-Transform Optical Photothermal Infrared Spectroscopy and Imaging.Analytical chemistry · 2025Article
- Single-Molecule Vibrational Thermometry.The journal of physical chemistry. B · 2025Article
- Two-dimensional bond-selective fluorescence spectroscopy: violations of the resonance condition, vibrational cooling rate dispersion, and super-multiplex imaging.Chemical science · 2025Article
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience.Biosensors · 2025Review
- Wide-field bond-selective fluorescence imaging: from single-molecule to cellular imaging beyond video rate.Optica · 2025Article
- Ultrasensitive infrared spectroscopy via vibrational modulation of plasmonic scattering from a nanocavity.Science advances · 2024Article
- Room-Temperature Single-Molecule Infrared Imaging and Spectroscopy through Bond-Selective Fluorescence.Angewandte Chemie (International ed. in English) · 2024Article
- INSPIRE: Single-beam probed complementary vibrational bioimaging.Science advances · 2024Article
- Surpassing the Diffraction Limit in Label-Free Optical Microscopy.ACS photonics · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Bioimaging harnessing optical contrasts and chemical specificity is of vital importance in probing complex biology. Vibrational spectroscopy based on mid-infrared (mid-IR) excitation can reveal rich chemical information about molecular distributions. However, its full potential for bioimaging is hindered by the achievable sensitivity. Here, we report bond selective fluorescence-detected infrared-excited (BonFIRE) spectral microscopy. BonFIRE employs two-photon excitation in the mid-IR and near-IR to upconvert vibrational excitations to electronic states for fluorescence detection, thus encoding vibrational information into fluorescence. The system utilizes tuneable narrowband picosecond pulses to ensure high sensitivity, biocompatibility, and robustness for bond-selective biological interrogations over a wide spectrum of reporter molecules. We demonstrate BonFIRE spectral imaging in both fingerprint and cell-silent spectroscopic windows with single-molecule sensitivity for common fluorescent dyes. We then demonstrate BonFIRE imaging on various intracellular targets in fixed and live cells, neurons, and tissues, with promises for further vibrational multiplexing. For dynamic bioanalysis in living systems, we implement a high-frequency modulation scheme and demonstrate time-lapse BonFIRE microscopy of live HeLa cells. We expect BonFIRE to expand the bioimaging toolbox by providing a new level of bond-specific vibrational information and facilitate functional imaging and sensing for biological investigations.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.