Evidence map›Paper›PMID 38159155›Full record

ArticleMikrochimica acta2023

Development of competitive and noncompetitive lateral flow immunoassays for pendimethalin using synthetic peptides.

Lianrun Huang, Guixiu Wang, Yanling Wu, Zhijia Wang, Yuan Ding, Hongwu Liang, Xiude Hua

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 8 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Lianrun HuangCollege of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Guixiu WangCollege of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Yanling WuCollege of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Zhijia WangCollege of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Yuan DingCollege of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Hongwu LiangInner Mongolia Key Laboratory of Environmental Pollution Control & Waste Resource Reuse, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, China.
Xiude HuaCollege of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China. huaxiude@njau.edu.cn.ORCID 0000-0003-0557-1892
Nanjing Agricultural University · CNInner Mongolia University · CN

Funding

Inner Mongolia University of Science and Technology Plan 2022YFSH0050Jiangsu Agriculture Science and Technology Innovation Fund CX(22)3075
6 · The paper itself

Abstract

Peptidomimetic and anti-immunocomplex peptides can be easily isolated from phage display libraries, and can be used as alternatives to chemical competing haptens to improve the sensitivity of small molecule immunoassay. In this work, 16 peptidomimetics and 7 anti-immunocomplex peptides of pendimethalin (PND) were obtained from cyclic 7-, 8-, 9-, and 10-residue peptide phage libraries. Peptidomimetic EJ-2 (CMFTGTDFPC) with the highest sensitivity in competitive phage enzyme-linked immunosorbent assay (ELISA) and immunocomplex peptide EF-30 (CNPGWPPIPC) with the highest sensitivity in noncompetitive phage ELISA were selected to prepare phage-free peptides with GGGSSK-biotin at the C-terminus. Competitive and noncompetitive lateral flow immunoassays (CLFIA and NLFIA) were developed by using the phage-free peptides. After optimization, the CLFIA and NLFIA showed visual limit of detections (vLODs) of 5 ng/mL and 2.5 ng/mL, respectively, which were improved two- and fourfold compared with a LFIA based on chemical hapten. The NLFIA showed better sensitivity than CLFIA in the detection of spiked samples, and can meet the detection requirements for agro-products regulated by EU and China. The detection results of CLFIA and NLFIA for blind samples were consistent with that of ultra performance liquid chromatography/tandem mass spectrometry.

Indexed as

BacteriophagesPeptidomimeticsAniline CompoundsEnzyme-Linked Immunosorbent AssayImmunoassayPeptide LibraryPeptidesAniline CompoundspendimethalinPeptide LibraryPeptidesPeptidomimeticsLater flow immunoassayPendimethalinPhage-display peptidesSmall molecule pollutant

Identifiers

PMID38159155
OpenAlexW4390444512

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.