Evidence map›Paper›PMID 38158490›Full record

ArticleApplied biochemistry and biotechnology2024

Preparation, Structural Characterization, and Enzymatic Properties of Alginate Lyase Immobilized on Magnetic Chitosan Microspheres.

Jinmeng Li, Feng Yan, Bingbing Huang, Mengyan Zhang, Xiaodan Wu, Yuhuan Liu, Roger Ruan, Hongli Zheng

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Jinmeng LiCollege of Food Science and Technology and International Institute of Food Innovation and State Key Laboratory of Food Science and Resources, Nanchang University, 235 East Nanjing Road, Nanchang, Jiangxi, 330047, People's Republic of China.
Feng YanCollege of Food Science and Technology and International Institute of Food Innovation and State Key Laboratory of Food Science and Resources, Nanchang University, 235 East Nanjing Road, Nanchang, Jiangxi, 330047, People's Republic of China.
Bingbing HuangCollege of Food Science and Technology and International Institute of Food Innovation and State Key Laboratory of Food Science and Resources, Nanchang University, 235 East Nanjing Road, Nanchang, Jiangxi, 330047, People's Republic of China.
Mengyan ZhangCollege of Food Science and Technology and International Institute of Food Innovation and State Key Laboratory of Food Science and Resources, Nanchang University, 235 East Nanjing Road, Nanchang, Jiangxi, 330047, People's Republic of China.
Xiaodan WuCollege of Food Science and Technology and International Institute of Food Innovation and State Key Laboratory of Food Science and Resources, Nanchang University, 235 East Nanjing Road, Nanchang, Jiangxi, 330047, People's Republic of China.
Yuhuan LiuCollege of Food Science and Technology and International Institute of Food Innovation and State Key Laboratory of Food Science and Resources, Nanchang University, 235 East Nanjing Road, Nanchang, Jiangxi, 330047, People's Republic of China.
Roger RuanCenter for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave, St. Paul, MN, 55108, USA.
Hongli ZhengCollege of Food Science and Technology and International Institute of Food Innovation and State Key Laboratory of Food Science and Resources, Nanchang University, 235 East Nanjing Road, Nanchang, Jiangxi, 330047, People's Republic of China. honglizheng@ncu.edu.cn.ORCID http://orcid.org/0000-0002-6484-4216
Nanchang University · CNUniversity of Minnesota · US

Funding

National Natural Science Foundation of China 21767017National Natural Science Foundation of China 22066016Science and Technology Innovation Talent Project of Jiangxi Province 20192BBH80023Science and Technology Innovation Talent Project of Jiangxi Province 20212BDH81004
6 · The paper itself

Abstract

Alginate lyase is an enzyme that catalyses the hydrolysis of alginate into alginate oligoalginates. To enhance enzyme stability and recovery, a facile strategy for alginate lyase immobilization was developed. Novel magnetic chitosan microspheres were synthesized and used as carriers to immobilize alginate lyase. The immobilization of alginate lyase on magnetic chitosan microspheres was successful, as proven by Fourier transform infrared spectroscopy and X-ray diffraction spectra. Enzyme immobilization exhibited the best performance at an MCM dosage of 1.5 g/L, adsorption time of 2.0 h, glutaraldehyde concentration of 0.2%, and immobilization time of 2.0 h. The optimal pH of the free alginate lyase was 7.5, and this pH value was shifted to 8.0 after immobilization. No difference was observed at the optimal temperature (45 °C) for the immobilized and free enzymes. The immobilized alginate lyase displayed better thermal stability than the free alginate lyase. The K

Indexed as

ChitosanEnzymes, ImmobilizedEnzyme StabilityMicrospheresPolysaccharide-LyasesAlginatesHydrogen-Ion ConcentrationKineticsTemperatureAlginatesChitosanEnzymes, Immobilizedpoly(beta-D-mannuronate) lyasePolysaccharide-LyasesAlginate lyaseEnzyme recoveryEnzyme stabilityImmobilizationMagnetic chitosan microspheres

Identifiers

PMID38158490
OpenAlexW4390430423

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.