Green Process for Producing Xylooligosaccharides by Using Sequential Auto-hydrolysis and Xylanase Hydrolysis.

Xylooligosaccharides (XOS), as prebiotic oligomers, are increasingly receiving attention as high value-added products produced from lignocellulosic biomass. Although the XOS contains a series of different degrees of polymerization (DP) of xylose units, DP 2 and 3 (xylobiose (X2) and xylotriose (X3)) are regarded as the main active components in food and pharmaceutical fields. Therefore, in the study, in order to achieve the maximum production of XOS with the desired DP, a combination strategy of sequential auto-hydrolysis and xylanase hydrolysis was developed with corncob as raw material. The evidences showed that the hemicellulosic xylan could be effectively decomposed into various higher DP saccharides (> 4), which were dissolved into the auto-hydrolysate; sequentially, the soluble saccharides could be rapidly hydrolyzed into XOS with desired DP by xylanase hydrolysis. Finally, a maximum XOS yield of 56.3% was achieved and the ratio of (X2 + X3)/XOS was over 80%; meanwhile, the by-products could be controlled at lower levels. Overall, this study provides solid data that support the selective and precise preparation of XOS from corncob, vigorously promoting the application of XOS as functional sugar products.