Evidence map›Paper›PMID 38153629›Full record

ArticleCurrent medical science2023

LOXL3 Inhibits Autophagy of Chondrocytes by Activating Rheb in Osteoarthritis.

Guang-Ping Zheng, Chen Liu, Liang Zhang, Qiang Zhong, Yun Zhang, Zhong-Ming Huang

Abstract read
PubMed Publisher
In one paragraph

Article in Current medical science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Guang-Ping Zheng *Ganzhou Municipal Key Laboratory of Bone and Joint Research, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, 341000, China.
Chen Liu *Ganzhou Municipal Key Laboratory of Bone and Joint Research, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, 341000, China.
Liang Zhang *Research Center of Translational Medicine, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan, 250013, China.
Qiang ZhongGanzhou Municipal Key Laboratory of Bone and Joint Research, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, 341000, China.
Yun ZhangGanzhou Municipal Key Laboratory of Bone and Joint Research, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, 341000, China.
Zhong-Ming HuangGanzhou Municipal Key Laboratory of Bone and Joint Research, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, 341000, China. 11118097@zju.edu.cn.
Ganzhou People's Hospital · CNNanchang University · CNJinan Central Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis study aimed to investigate the potential mechanisms by which lysyl oxidase like 3 (LOXL3) affects the autophagy in chondrocytes in osteoarthritis (OA), specifically through the activation of mammalian target of rapamycin complex 1 (mTORC1).

methodsTo establish an OA model, rats underwent anterior cruciate ligament transection (ACLT). Chondrocytes were isolated from cartilage tissues and cultured. Western blotting was performed to assess the expression of LOXL3, Rheb, phosphorylation of p70S6K (p-p70S6K, a downstream marker of mTORC1), and autophagy markers. The autophagy of chondrocytes was observed using an immunofluorescence assay.

resultsThe expression levels of both LOXL3 and Rheb proteins were upregulated in chondrocytes isolated from the OA model cartilage, in comparison to those from the normal cartilage. The silencing of LOXL3 resulted in a decrease in the protein levels of Rheb and p-p70S6K, as well as an increase in the expression of autophagy-related proteins. Additionally, the effect of LOXL3 could be reversed through the silencing of Rheb. The results of the immunofluorescence assay confirmed the impact of LOXL3 and Rheb on chondrocyte autophagy.

conclusionLOXL3 inhibits chondrocyte autophagy by activating the Rheb and mTORC1 signaling pathways.

Indexed as

Amino Acid OxidoreductasesChondrocytesOsteoarthritisAnimalsAutophagyMammalsMechanistic Target of Rapamycin Complex 1Ras Homolog Enriched in Brain ProteinRatsRibosomal Protein S6 Kinases, 70-kDaAmino Acid OxidoreductasesLoxl3 protein, ratMechanistic Target of Rapamycin Complex 1Ras Homolog Enriched in Brain ProteinRheb protein, ratRibosomal Protein S6 Kinases, 70-kDaautophagychondrocyteLOXL3mTORC1osteoarthritisRheb

Identifiers

PMID38153629
OpenAlexW4390347931

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.