Evidence map›Paper›PMID 38147222›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

In Vitro Phosphatase Assays for the Eya2 Tyrosine Phosphatase.

Christopher Alderman, Aaron Krueger, John Rossi, Heide L Ford, Rui Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Christopher AldermanDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Aaron KruegerDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
John RossiDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Heide L FordDepartment of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Rui ZhaoDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA. rui.zhao@cuanschutz.edu.

Funding

The molecular mechanism of pre-mRNA splicingR35GM145289 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI RUI ZHAO · 2022 to 2026
$4.3M
Training in Translational Research of Lung, Head and Neck CancerT32CA174648 · NCI · UNIVERSITY OF COLORADO DENVER · PI KARAM, SANA D · 2013 to 2022
$4.2M
Deciphering Mechanisms by which Tumor Cells Collaborate to Mediate MetastasisR01CA224867 · NCI · UNIVERSITY OF COLORADO DENVER · PI FORD, HEIDE L., LEWIS, MICHAEL T. · 2019 to 2023
$3.2M
Role of Eya3 in regulating the immune microenvironment to promote breast tumor progressionR01CA221282 · NCI · UNIVERSITY OF COLORADO DENVER · PI FORD, HEIDE L., ZHAO, RUI · 2017 to 2021
$3.2M
Examining the EYA2/MYC axis in Group 3 MedulloblastomaR01NS108396 · NINDS · UNIVERSITY OF COLORADO DENVER · PI FORD, HEIDE L. · 2018 to 2021
$1.6M
The anti-tumorigenic and anti-metastatic potential of Eya phosphatase inhibitorsR41CA180347 · NCI · SIXONE SOLUTIONS, LLC · PI FORD, HEIDE L., ZHAO, RUI · 2013 to 2013
$293k
Identify inhibitors of the Eya phosphatase activity using high throughput screeniR03DA030559 · NIDA · UNIVERSITY OF COLORADO DENVER · PI FORD, HEIDE L., ZHAO, RUI · 2010 to 2011
$74k
NCI NIH HHS R01 CA221282NCI NIH HHS R01 CA224867NCI NIH HHS R41 CA180347NCI NIH HHS T32 CA174648NIDA NIH HHS R03 DA030559NIGMS NIH HHS R35 GM145289NINDS NIH HHS R01 NS108396
6 · The paper itself

Abstract

Protein tyrosine phosphatases (PTP), such as the Eyes Absent (Eya) family of proteins, play important roles in diverse biological processes. In vitro phosphatase assays are essential tools for characterizing the enzymatic activity as well as discovering inhibitors and regulators of these phosphatases. Two common types of in vitro phosphatase assays use either a small molecule substrate that produces a fluorescent or colored product, or a peptide substrate that produces a colorimetric product in a malachite green assay. In this chapter, we describe detailed protocols of a phosphatase assay using small molecule 3-O-methylfluorescein phosphate (OMFP) as a substrate and a malachite green assay using the pH2AX peptide as a substrate to evaluate the phosphatase activity of EYA2 and the effect of small molecule inhibitors of EYA2. These protocols can be easily adapted to study other protein tyrosine phosphatases.

Indexed as

Protein Tyrosine PhosphatasesRosaniline DyesPeptidesTyrosinemalachite greenPeptidesProtein Tyrosine PhosphatasesRosaniline DyesTyrosineEya2Malachite green assayOMFPPhosphatase assayProtein tyrosine phosphatase

Identifiers

PMID38147222
PMCPMC11044973

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.