Evidence map›Paper›PMID 38147219›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Measuring the Reversible Oxidation of Protein Tyrosine Phosphatases Using a Modified Cysteinyl-Labeling Assay.

Avinash D Londhe, Benoit Boivin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Avinash D LondheDepartment of Nanobioscience, College of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, NY, USA.
Benoit BoivinDepartment of Nanobioscience, College of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, NY, USA. bboivin@albany.edu.

Funding

Role of Protein Tyrosine Phosphatase 1B in Cardiac HypertrophyR01HL175391 · NHLBI · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI Benoit Boivin · 2024 to 2026
$1.6M
Complexity of Protein Tyrosine Phosphatase OxidationR35GM153449 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI Benoit Boivin · 2024 to 2026
$1.1M
Role of the Phosphoprotein Phosphatase PTP1B in Cardiac HypertrophyR15HL138605 · NHLBI · SUNY POLYTECHNIC INSTITUTE · PI BOIVIN, BENOIT · 2017 to 2020
$896k
NHLBI NIH HHS R01 HL175391NHLBI NIH HHS R15 HL138605NIGMS NIH HHS R35 GM153449
6 · The paper itself

Abstract

The modified cysteinyl-labeling assay enables the labeling, enrichment, and detection of all members of the protein tyrosine phosphatase (PTP) superfamily that become reversibly oxidized in cells to facilitate phosphorylation-dependent signaling. In this chapter, we describe the method in detail and highlight the pitfalls of avoiding post-lysis oxidation of PTPs to measure the dynamic and transient oxidation and reduction of PTPs in cell signaling.

Indexed as

Protein Tyrosine PhosphatasesSignal TransductionBiological AssayCell DeathOxidation-ReductionProtein Tyrosine PhosphatasesBiotin labelingProtein tyrosine phosphatasesRedox signalingReversible oxidationSulfhydryl reactive probes

Identifiers

PMID38147219
PMCPMC13112361

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.