Evidence map›Paper›PMID 38147211›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Examining Phosphatases Through Immunofluorescent Microscopy.

Caroline N Smith, Jessica S Blackburn

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Caroline N SmithMolecular and Cellular Biochemistry Department, University of Kentucky, Lexington, KY, USA.
Jessica S BlackburnMolecular and Cellular Biochemistry Department, University of Kentucky, Lexington, KY, USA. jsblackburn@uky.edu.

Funding

The Phosphatase PRL3 as a MYC Target and Pro-Survival Oncogene in Acute Lymphoblastic LeukemiaR37CA227656 · NCI · UNIVERSITY OF KENTUCKY · PI BLACKBURN, JESSICA S. · 2018 to 2024
$2.4M
6 · The paper itself

Abstract

Immunofluorescent microscopy enables the examination of cellular expression and localization of proteins. Cellular localization can often impact protein function, as certain molecular interactions occur in specific cellular compartments. Here we describe in detail the processes necessary for identifying phosphatases in the cell through immunofluorescent microscopy. Identification of phosphatase expression and localization could lead to the discovery of protein function in disease states along with potential substrates and binding partners.

Indexed as

Phosphoric Monoester HydrolasesMicroscopy, FluorescencePhosphoric Monoester HydrolasesAntibodyConfocalImmunofluorescenceLocalizationMicroscopyProtein expression

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.