Evidence map›Paper›PMID 38147207›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

CRISPR/Cas9-Mediated Modification of PTP Expression.

Carolin Lossius, Anne Kresinsky, Laura Quiet, Jörg P Müller

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Carolin Lossius *Institute for Molecular Cell Biology, CMB - Center for Molecular Biomedicine; University Hospital Jena, Jena, Germany.
Anne Kresinsky *Institute for Molecular Cell Biology, CMB - Center for Molecular Biomedicine; University Hospital Jena, Jena, Germany.
Laura QuietInstitute for Molecular Cell Biology, CMB - Center for Molecular Biomedicine; University Hospital Jena, Jena, Germany.
Jörg P MüllerInstitute for Molecular Cell Biology, CMB - Center for Molecular Biomedicine; University Hospital Jena, Jena, Germany. joerg.mueller@med.uni-jena.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alteration of protein tyrosine phosphatase (PTP) gene expression is a commonly used approach to experimentally analyze their function in the cell physiology of mammalian cells. Here, exemplified for receptor-type PTPRJ (Dep-1, CD148) and PPTRC (CD45), we provide the CRISPR/Cas9-mediated approaches for their inactivation and transcriptional activation using genome editing. These methods are generally applicable to any other protein of interest.

Indexed as

CRISPR-Cas SystemsGene EditingAnimalsMammalsTranscriptional ActivationCas9CRISPRFlow cytometryGene expressionImmune detectionNonhomologous end joiningProtein tyrosine phosphataseSynergistic activation mediators

Identifiers

PMID38147207

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.