Evidence map›Paper›PMID 38141873›Full record

ArticleJournal of molecular biology2024

SwrA-mediated Multimerization of DegU and an Upstream Activation Sequence Enhance Flagellar Gene Expression in Bacillus subtilis.

Ayushi Mishra, Anna C Hughes, Jeremy D Amon, David Z Rudner, Xindan Wang, Daniel B Kearns

Open access · greenAbstract read
In one paragraph

Article in Journal of molecular biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. The last bacterial common ancestor encoded a complex flagellum.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. The extracytoplasmic sigma factor σScientific reports · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Ayushi MishraDepartment of Biology, Indiana University, Bloomington, IN 47408, USA.
Anna C HughesDepartment of Biology, Indiana University, Bloomington, IN 47408, USA.
Jeremy D AmonDepartment of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
David Z RudnerDepartment of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
Xindan WangDepartment of Biology, Indiana University, Bloomington, IN 47408, USA.
Daniel B KearnsDepartment of Biology, Indiana University, Bloomington, IN 47408, USA. Electronic address: dbkearns@indiana.edu.
Indiana University Bloomington · USHarvard University · US

Funding

Swarming motility and the regulation of flagellar biosynthesis in Bacillus subtilisR35GM131783 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI Daniel B Kearns · 2019 to 2026
$3.9M
Growth and differentiation in Bacillus subtilisR35GM145299 · NIGMS · HARVARD MEDICAL SCHOOL · PI DAVID Z RUDNER · 2022 to 2026
$2.9M
Physicochemical properties driving membraneless organelle assembly in bacteriaR01GM143182 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BITEEN, JULIE, MEYER, ANNE SARA · 2021 to 2024
$2.4M
Determining the molecular basis of gene silencing by MucR and defining its role in Brucella virulenceR01AI172822 · NIAID · EAST CAROLINA UNIVERSITY · PI ROY M ROOP · 2023 to 2026
$2.3M
How bacterial SMC complexes organize chromosomes (Equipment Supplement)R01GM141242 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI WANG, XINDAN · 2021 to 2025
$1.8M
NIAID NIH HHS R01 AI172822NIGMS NIH HHS R01 GM141242NIGMS NIH HHS R01 GM143182NIGMS NIH HHS R35 GM131783NIGMS NIH HHS R35 GM145299
6 · The paper itself

Abstract

The earliest genes in bacterial flagellar assembly are activated by narrowly-conserved proteins called master regulators that often act as heteromeric complexes. A complex of SwrA and the response-regulator transcription factor DegU is thought to form the master flagellar regulator in Bacillus subtilis but how the two proteins co-operate to activate gene expression is poorly-understood. Here we find using ChIP-Seq that SwrA interacts with a subset of DegU binding sites in the chromosome and does so in a DegU-dependent manner. Using this information, we identify a DegU-specific inverted repeat DNA sequence in the P

Indexed as

Bacillus subtilisBacterial ProteinsGene Expression Regulation, BacterialTranscription FactorsPhosphorylationPromoter Regions, GeneticProtein MultimerizationBacterial ProteinsDegU protein, BacteriaTranscription FactorsflagellaSwrAtranscription activationtwo-component systemUP element

Identifiers

PMID38141873
PMCPMC11462632
OpenAlexW4390047467

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.