Evidence map›Paper›PMID 38140077›Full record

ArticlePharmaceutics2023

Investigation on Electrospun and Solvent-Casted PCL-PLGA Blends Scaffolds Embedded with Induced Pluripotent Stem Cells for Tissue Engineering.

Mariella Rosalia, Martina Giacomini, Erika Maria Tottoli, Rossella Dorati, Giovanna Bruni, Ida Genta, Enrica Chiesa, Silvia Pisani, Maurilio Sampaolesi, Bice Conti

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceutics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 6 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Mariella RosaliaDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.ORCID 0000-0002-3677-3393
Martina GiacominiDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
Erika Maria TottoliDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
Rossella DoratiDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.ORCID 0000-0001-5774-9547
Giovanna BruniConsorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase (C.S.G.I.), Department of Chemistry, Physical Chemistry Section, University of Pavia, Via Taramelli 10, 27100 Pavia, Italy.ORCID 0000-0003-1958-2998
Ida GentaDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.ORCID 0000-0001-5710-0588
Enrica ChiesaDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
Silvia PisaniDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.ORCID 0000-0002-5396-1601
Maurilio SampaolesiTranslational Cardiomyology Laboratory, Head Unit of Stem Cell and Developmental Biology (SCDB), Head Department of Development and Regeneration, KU Leuven, ON4 Herestraat 49, Box 804, 3000 Leuven, Belgium.ORCID 0000-0002-2422-3757
Bice ContiDepartment of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.ORCID 0000-0002-0034-2815
University of Pavia · ITKU Leuven · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The design, production, and characterisation of tissue-engineered scaffolds made of polylactic-co-glycolic acid (PLGA), polycaprolactone (PCL) and their blends obtained through electrospinning (ES) or solvent casting/particulate leaching (SC) manufacturing techniques are presented here. The polymer blend composition was chosen to always obtain a prevalence of one of the two polymers, in order to investigate the contribution of the less concentrated polymer on the scaffolds' properties. Physical-chemical characterization of ES scaffolds demonstrated that tailoring of fibre diameter and Young modulus (YM) was possible by controlling PCL concentration in PLGA-based blends, increasing the fibre diameter from 0.6 to 1.0 µm and reducing the YM from about 22 to 9 MPa. SC scaffolds showed a "bubble-like" topography, caused by the porogen spherical particles, which is responsible for decreasing the contact angles from about 110° in ES scaffolds to about 74° in SC specimens. Nevertheless, due to phase separation within the blend, solvent-casted samples displayed less reproducible properties. Furthermore, ES samples were characterised by 10-fold higher water uptake than SC scaffolds. The scaffolds suitability as iPSCs culturing support was evaluated using XTT assay, and pluripotency and integrin gene expression were investigated using RT-PCR and RT-qPCR. Thanks to their higher wettability and appropriate YM, SC scaffolds seemed to be superior in ensuring high cell viability over 5 days, whereas the ability to maintain iPSCs pluripotency status was found to be similar for ES and SC scaffolds.

Indexed as

electrospinningiPSCpolymer blendssolvent casting/particulate leaching

Identifiers

PMID38140077
PMCPMC10747843
OpenAlexW4389388475

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.